ANTIBACTERIAL ACTIVITY OF INDIGENOUS ENTOMOPATHOGENIC FUNGUS METARHIZIUM ANISOPLIAE AGAINST CLINICALLY ISOLATED HUMAN PATHOGENS
Keywords:Entomopathogens, M anisopliae, Human pathogens, Bioactive compound, HPLC
Objectives: The aim of this study was to determine the bactericidal effect of indigenously isolated entomopathogenic fungus Metarhizium anisopliae against clinically isolated human pathogens such as Bacillus subtills, Pseudomonas spp, Proteus spp, Staphylococcus aureus, E.coli, Shigella spp, Entrocooci spp.
Methods: The isolated fungus was mass cultivated in liquid state fermentation and the extracellular products were extracted with dichloromethane. With the support of preparative high performance liquid chromatography (HPLC) the extract was separated and the active metabolites were characterized. Further, the extract was tested for its bactericidal efficacy against clinically isolated human pathogens via well diffusion method. Briefly, different concentrations of the extracts were loaded onto the bacterial culture growing in nutrient agar plates.
Results: Formation of clear zones after 24h of incubation indicates the significant inhibitory action of active metabolites present in the three different concentrations (25Âµl, 50Âµl, 75Âµl) of crude extract. High level exhibit best inhibitory activity was found against B.subtills (26.4Â±0.1),
Conclusion: The findings of this preliminary study gives overview idea about the differential anti-bacterial property of M. anisopliae and additionally it will help us to find specific antibiotic products for biomedical applications.
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