STUDY THE RELATIONSHIP BETWEEN ANTIOXIDANT POTENTIAL AND PHENOLIC CONTENTS OF JUNIPERUS EXCELSA FRUIT
Keywords:Juniperus excelsa fruit, Fractions, Reducing power, DPPH radical scavenging Phenolic contents
Objective: Today's attentions are focused on the finding new natural antioxidant compounds because of their fewer side effects than synthetic antioxidants. The aim of this study was to determine the antioxidant potentials of Juniperus excelsa fruit and its fractions by different methods.
Methods: For evaluation antioxidant potentials, DPPH radical scavenging, determination of reducing power and phenolics were used. Gallic acid and quercetin were used as antioxidant standards.
Results: The highest DPPH radical scavenging was observed in n-butanol fraction (IC 50= 135.9Â±2.5 Âµg/ml) of Juniperus excelsa fruit. Also, this fraction possesses the highest reducing power (in 61.4Â±2.6 Âµg/ml with absorbance 0.5) and phenolic contents (82. 9Â±1.1 mg/g).
Conclusion: n-butanol fraction of Juniperus excelsa fruit had the highest radical scavenging, reducing power and phenolic compounds. In other words, a relationship between antioxidant potentials and phenolic compounds was found. Anyway, this fraction is a strong source of antioxidant compounds and can be used as a natural antioxidant.
Emami SA, Asili J, Mohagheghi Z, Hassanzadeh MK. Antioxidant activity of leaves and fruits of Iranian conifers. Evidence-based complementary and alternative medicine:eCAM 2007; 4(3): 313-9.
Yu L, Zhao M, Yang B, Bai W. Immunomodulatory and anticancer activities of phenolics from Garcinia mangostana fruit pericarp. Food Chem 2009; 116:969-973.
Orhan N, Orhan IE, Ergun F. Insights into cholinesterase inhibitory and antioxidant activities of five Juniperus species. Food and chemical toxicology:an international journal published for the British Industrial Biological Research Association 2011;49(9):2305-12.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int Cell 7;39:44-84.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160(1):1-40.
Moein S, Moein MR, Ahmadizadeh S. Antioxidant activity and phenolic content of Juniperus excelsa extract. IJPS 2010; 6(2): 133-140.
Moein S, Moein MR, Khoshnoud, Kalantari T. In vitro antioxidant properties evaluation of 10 Iranian medicinal plants by different methods. Iran Red Crescent Med J 2012; 14(12):771-775.
Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal plants and aromatic plant extract. Food Chem 2004; 85: 231-237.
ElmastaÅŸ M, GÃ¼lÃ§in I, Beydemir Åž, KÃ¼frevioÇ§lu Ã–I, Aboul-Enein HY. A study on the in vitro antioxidan activity of juniperus (Juniperus communis L) fruit extracts. Anal Lett 2006; 3: 47-65.
El-Ghorab A, Shaaban HA, El-Massry KF, Shibamoto T. Chemical composition of volatile extract and biological activities of volatile and less-volatile extracts of juniper berry (Juniperus drupacea L.) fruit. J Agric Food Chem 2008;56(13):5021-5.
Lesjak MM, Beara IN, Orcic DZ, Anackov GT, Balog KJ, Franciskovic MM. Mimica-DukiÄ‡ N:Juniperus sibirica Burgsdorf as a novel source of antioxidant and anti-inflammatory agents. Food Chem 2011;124:850-6.
Soares AA, de Souza CGM, Daniel FM, Ferrari GP, Costa SMG, Peralta RM. da Antioxidant activity and total phenolic content of Agaricus brasiliensis (Agaricus blazei Murril) in two stages of maturity. Food Chem 2009;112:775-81.
Ignat I, Volf I, Popa VI. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem 2011;126:1821-35.
Moein S, Farzami B, Khaghani S, Moein M, Larijani B. Antioxidant properties and prevention of cell cytotoxicity of Phlomis persica Boiss. Daru 7;15:83-8.
Gupta A, Srivastava S, Prasad R, Natu SM, Mittal B, Negi MPS, et al. Smoking intensity, oxidative stress and chemotherapy in nonsmall cell lung cancer:a correlated prognostic study. Biosci Trends 2009;3(5):191-9.
Jong PL, Young CS, Jin WK, Chung HK, Jae SE, Kang HL. Free radical scavengers from the heartwood of Juniperus chinensis. Arch Pharm Res 2002;25:449-52.
Moein MR, Ghasemi Y, Moein S, Nejati M. Analysis of antimicrobial, antifungal and antioxidant activities of Juniperus excelsa M. B subsp. Polycarpos (K. Koch) Takhtajan essential oil. Pharmacognosy Res 2010;2(3):128-31.
Fang Y-Z, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition (Burbank, Los Angeles County, Calif) 2002;18(10):872-9.
Molyneux P, J. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Technol 2004;26:211-9