• Eng-soon Khor Biotechnology Programme, School of Science & Technology, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia.
  • Nyet-kui Wong Biotechnology Programme, School of Science & Technology, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia.


Carica Papaya, Antioxidant, Cytotoxicity, Total phenolic and favonoid content, Ethyl acetate fraction


Objective: This study was done to examine antioxidant and cytotoxic potential in the extracts of fruits and seeds from the traditionally used medicinal plant, Carica papaya.

Methods: Antioxidant and cytotoxic potential of crude methanol, hexane fraction and ethyl acetate fraction from both fruits and seeds of Carica papaya were compared and assessed by DPPH free-radical scavenging assay and brine shrimp lethality assay. Total phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride colorimetric methods respectively. Bioactive fractions were then characterized and analyzed by silica thin layer chromatography.

Results: Results showed that both ethyl acetate fractions from the fruits and seeds of Carica papaya are high in their antioxidant activities (IC50 values of 30.61 µg/ml and 25.97 µg/ml respectively) as well as cytotoxic (LC50 of 163.96 µg/ml and 142.27 µg/ml respectively). High antioxidant activities of ethyl acetate fractions obtained from fruits and seeds are strongly correlated to the total phenolic contents and moderately correlated to the total flavonoid contents.

Conclusion: This study suggested that both ethyl acetate fractions from fruits and seeds of Carica papaya may have the potential to be further developed into therapeutic option for treating cancer, of which the­ ethyl acetate fraction from seeds raises a slightly higher prospect.


Download data is not yet available.


Fadeyi SA, Fadeyi OO, Adejumo AA, Okoro C, Myles EL. In Vitro Anticancer Screening of 24 Locally Used Nigerian Medicinal Plants. BMC Complementary and Alternative Medicine 2013;13(79).

Savithramma N, Rao ML, Suhrulatha D. Screening of Medicinal Plants for Secondary Metabolites. Middle-East Journal of Scientific Research 2011;8(3):579-584.

Jaime da Silva AT, Rashid Z, Nhut DT, Sivakumar D, Gera A, Manoel Jr TS,Tennant PF. Papaya (Carica papaya L.) Biology and Biotechnology. Tree and Forestry Science and Biotechnology 2007;1(1):47-73.

Mozaffarieh M, Sacu S, Wedrich A. The Role of the Carotenoids, Lutein and Zeaxanthin, in Protecting Against Age Related Macular Degeneration:A Review Based on Controversial Evidence. Nutrition Journal 2003;2(20):1-8.

Rashed KN, Fouche G. Anticancer Activity of Carica papaya Extracts in Vito and Phytochemical Analysis. Greener Journal of Pharmacy and Pharmacology 2013;1(1):001-005.

Emeruwa AC. Antibacterial Substance from Carica papaya Fruit Extract. Journal of Natural Products 1982;45(2):123-127.

Dawkins G, Hewitt H, Wint Y, Obiefuna PC, Wint B. Antibacterial Effects of Carica papaya Fruit on Common Wound Organisms. West Indian Medical Journal 2003;52(4):290-292.

Lim YY, Lim TT, Tee JJ. Antioxidant Properties of Several Tropical Fruits:A Comparative Study. Food Chemistry 2007;103:1003-1008.

Isabelle M, Lee BL, Lim MT, Koh WP, Huang D, Ong CN. Antioxidant Activity and Profiles of Common Fruits in Singapore. Food Chemistry 2010;123:77-84.

Kothari V, Seshadri S. Antioxidant Activity of Seed Extracts of Annona squamosa and Carica papaya. Nutrition & Food Science 2010;40(4):403-408.

Zhou KB, Wang H, Mei WL, Li X, Luo Y, Dai HF. Antioxidant Activity of Papaya Seed Extracts. Molecules 2011;16(8):6179-6192.

Oloyede O, Roos D, Rocha J. Studies on the Antioxidant Potential of Extracts from Unripe Fruit of Carica papaya. Journal of Life Science 2012;6:41-47.

Maisarah AM, Nurul AB, Asmah R, Fauziah O. Antioxidant Analysis of Different Parts of Carica papaya. International Food Research Journal 2013;20(3):1043-1048.

Sadek KM. Antioxidant and Immunostimulant Effect of Carica papaya Linn. Aqueous Extract in Acrylamide Intoxicated Rats. Acta Informatica Medica 2012;20(3):180-185.

Dwikat M, Dini L. Antioxidant Effect of Aqueous Carica papaya Seed Extract. 2nd Conference on Biotechnology Research and Applications. 26-27 September 2010, An-Najah National University, Nablus, Palestine.

Alothman M, Rajeev B, Karim AA. Antioxidant Capacity and Phenolic Content of Selected Tropical Fruits from Malaysia, Extracted with Different Solvents. Food Chemistry 2009;115:785-788.

Norshazila S, Syed ZI, Mustapha SK, Aisyah MR, Kamarul RK. Antioxidant Levels and Activities of Selected Seeds of Malaysian Tropical Fruits. Malaysian Journal of Nutrition 2010;16(1):149-159.

Krishna KL, Paridhavi M, Jagruti AP. Review on Nutritional, Medicinal and Pharmacological Properties of Papaya (Carica papaya Linn.). Natural Product Report Journal 2008;7:364-373.

Kibria G, Hussain MM. In Vitro Cytotoxic Effect of Methanolic Crude Extracts of Terminalia chebula. International Research Journal of Pharmacy 2012;3(9):94-95.

Baskar R, Shrisakthi S, Sathyapriya B, Shayampriya R, Nithya R, Poongodi P. Antioxidant Potential of Peel Extracts of Banana Varieties (Musa sapientum). Food and Nutrition Sciences 2011;2:1128-1133.

Afidah HO, Rizal AR, Nornaemah R, Nurulafiqah MB, Nor HN. Tannin Content and Antioxidant Activity of Dried Plants Garcinia mangostana Linn. and Garcinia atroviridis Griff. In:Anders T, Haghi AK, editors. Food Science. Canada:Apple Academic Press;2011. p. 28-37.

Shahriar M, Hossain MI, Sharmin FA, Akhter S, Haque MA, Bhuiyan MA. In Vitro Antioxidant and Free Radical Scavenging Activity of Withania somniferaRoot. IOSR Journal of Pharmacy 2013;3(2):38-47.

Nurul SR, Asmah R. Evaluation of Antioxidant Properties in Fresh and Pickled Papaya. International Food Research Journal 2012;19(3):1117-1124.

Patil SB, Magdum CS. Determination of LC50 Values of Extracts of Euphorbia hirta Linn and Euphorbia neriifolia Linn. Using Brine Shrimp Lethality Assay. Asian Journal of Research in Pharmaceutical 2011;1(2):42-43.

Rieser MJ, Gu ZM, Fang XP, Zeng L, Wood KV, McLaughlin JL. Five Novel Monotetrahydrofuran Ring Acetogenins from the Seeds of Annona muricata. Journal of Natural Products 1996;59:100-108.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine Shrimp:A Convenient General Bioassay for Active Plant Constituents. Planta Medica 1982;45:31-34.

Costa TA, Vieira RF, Bizzo HR, Silveira D, Gimenes MA. Secondary Metabolites. In:Dhanarasu S, editors. C. Chromatography and Its Applications. Croatia:In Tech;2012, 131-164.



How to Cite

Khor, E.- soon, and N.- kui Wong. “POTENTIAL ANTIOXIDANT AND CYTOTOXIC PROPERTIES OF SECONDARY METABOLITE EXTRACTS FROM CARICA PAPAYA FRUITS AND SEEDS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 220-4, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1735.



Original Article(s)