DEVELOPMENT, VALIDATION OF HPTLC METHOD FOR SIMULTANEOUS QUANTITATION OF LUTEOLIN, APIGENIN FROM CARDIOSPERMUM HALICACABUM LINN. AND HYDNOCARPUS PENTANDRA (BUCH.-HAM.) OKEN
Keywords:Luteolin, apigenin, Cardiospermum halicacabum Linn, Hydnocarpus pentandra(Buch-Ham) Oken, High Performance Thin Layer Chromatography, Method validation
Objectives: To develop and validate High Performance Thin Layer Chromatographic (HPTLC) method for simultaneous quantitation of luteolin and apigenin from dried leaf powder of Cardiospermum halicacabum Linn. and dried seed hull powder of Hydnocarpus pentandra (Buch.-Ham.) Oken.
Methods: Chromatography was performed using methanolic extracts of dried leaf powder of Cardiospermum halicacabum Linn. and dried seed hull powder of Hydnocarpus pentandra (Buch.-Ham.) Oken. Separation of luteolin and apigenin from methanolic extracts of both the plant materials was achieved on Silica gel 60F254 TLC plates using a suitable solvent system. Detection and quantitation of luetolin and apigenin was done by densitometric scanning at Î»=349 nm. The developed HPTLC method has been validated using International Conference on Harmonization (ICH) guidelines.
Results: The validated HPTLC method was used for simultaneous quantitation of luteolin and apigenin from methanolic extracts of dried leaf powder of Cardiospermum halicacabum Linn. and dried seed hull powder of Hydnocarpus pentandra (Buch.-Ham.) Oken using their respective calibration curves. Amounts of luteolin and apigenin present in dried leaf powder of Cardiospermum halicacabum Linn. are 0.2119mg/g and 0.9089mg/g respectively. Amounts of luteolin and apigenin present in dried seed hull powder of Hydnocarpus pentandra (Buch.-Ham.) Oken are 0.0696mg/g and 0.0320mg/g.
Conclusion: The developed method is simple, precise and accurate and can also be used for routine quality control analysis and for the quantitation of luteolin and apigenin in herbal raw materials as well as in their formulations.
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