DETERMINATION OF PHYTOCOMPOUNDS FROM TERMINALIA CHEBULA RETZ BY HPTLC DENSITOMETRIC METHOD

Authors

  • Savitha T. PG & Research Department of Microbiology, KSR College of Arts & Science, Tiruchengode, Tamilnadu, India. Department of Microbiology, Tiruppur Kumaran College for Women, Tiruppur, Tamilnadu, India.
  • Arivukkarasu R. Department of Pharmaceutical Analysis, KMCH College of Pharmacy, Coimbatore. Tamilnadu, India.

Keywords:

HPTLC, Densitometry, Ellagic acid, Tannic acid

Abstract

Objective: The present investigation has been focused on the detection of antibacterial activity of methanolic extract by disc diffusion method and the quantitative estimation of phyto constituents from Terminalia chebula, (King of Medicine) by High Performance Thin Layer Chromatography (HPTLC) method.

Methods: An in vitro study on the efficacy of methanol extract of T.chebula was carried out. For this analysis, Tannic acid (TA), Gallic acid (GA), Ellagic acid (EA) were as used as standard markers by using toluene: ethyl acetate: formic acid: methanol (4.3:4.3:1:1.2:0.3, V/V/V/V) was used as a mobile phase. Detection and quantification were performed densitometrically at Lambda 254 nm.

Results: The methanol extract has shown best activity against test strains. The Rf values of standards were 0.78 for TA, 0.74 for GA and 0.63 for EA. The total peak areas of the standards and the corresponding peak areas of extracts were composed and the statistical analysis was carried out.

Conclusions: Based on the present findings, there is a wide opportunity for the development of new drug formulations for the effective treatment against multiple drug resistant micro organisms with no side effects with lesser costs.

Downloads

Download data is not yet available.

References

Modi KP, Patel NM, Goyal RK. Estimation of L-dopa from Mucuna pruriens LINN and formulations containing M. pruriens by HPTLC method. Chem Pharm Bull (Tokyo) 2008;56(3):357-9.

Hussain G, Mishra D, Singh P, Rao C, Review T, Kumar V. M, N, V, PHCOG REV:Plant chebula:an update. Pharmacogn Rev 2007;1(1):19-29.

Han Q, Song J, Qiao C, Wong L, Xu H. Preparative isolation of hydrolysable tannins chebulagic acid and chebulinic acid from Terminalia chebula by high-speed counter-current chromatography. Journal of separation science 2006;29(11):1653-7.

Lewis K, Ansubel FM. Prospects for plant derived antibacterials. Nat Biotechnol 2003;24:1504-7.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.

Lopes GK, Schulman HM, Hermes-Lima M. Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions. Biochim Biophys Acta 1999;1472(1-2):142-52.

Laksman K, Jayaveera K, Tripathi SNM, Satish KV. Kumar.A, N, Estimation of Gallic acid, Rutin and Quarcetin in Terminalia chebula by HPTLC. Jordan Journal of Pharmaceutical Sciences 2010;3(1):63-8.

Yoshimura M, Watanabe Y, Kasai K, Yamakoshi J, Koga T. Inhibitory effect of an ellagic acid-rich pomegranate extract on tyrosinase activity and ultraviolet-induced pigmentation. Biosci Biotechnol Biochem 2005;69(12):2368-73.

Mahajan A, Pai NR. D and Development and validation of HPLC method for quantification of phytoconstituents in Haritaki Churna. IntJ of ChemTech Research ISSN 09744290 2011;3(1):329-36.

Bag A, Bhattacharyya SK, Bharati P, Pal NK, Chattopadhyay RR. Antibacterial activity of Chebulic myrobalan (fruit of Terminalia chebula Retz. extracts against methicillin resistant Staphylococcus aureus and trimethoprimsulphamethoxazole resistant uropathogenic Escherichia coli Afr J Plant Sci 2009;3(2):25-9.

Rajasekaran A, Arivukkarasu R, Archana D. HPTLC method for estimation of Gallic acid and Rutin in Haritaki-An Ayurvedic formulation. International Journal of Pharm Tech Research ISSN 09744304 2011;3(2):986-99.

Kalyani R, Komal K, Shukla VJ, Prajapati PK. A study to evaluate ‘Diosgenin’ in Loghu gokshur (T.terrestries) and Brihat gokshur (Pedalium murex) by HPTLC method. International Journal of Pharmaceutical and Biological Archives. 2012;3 (5):1117–20. ISSN:0976 – 3333.

Mukherjee PK. Problems and prospects for the GMP in herbal drugs in Indian systems of medicine. Drug information Journal a 2002;63(3):635-44.

Yao J, Moellering R, Murray P, Baron E, Pfaller M, Tenover F, et al. Antibacterial agents. In eds Manual of clinical microbiology ASM 1995:1281-90.

Mimica-Dukic N, Kujundzic S, Sokovic M, Couladis M. Essential oil composition and antifungal activity of Foeniculum vulgare Mill obtained by different distillation conditions. Phytother Res 2003;17(4):368-71.

Meng JC, Zhu QX, Tan RX. New antimicrobial mono-and sesquiterpenes from Soroseris hookeriana subsp. erysimoides. Planta Med 2000;66(6):541-4.

Balasaraswathi R, Sadasivam S, Ward M, Walker JM. An antiviral protein from Bougainvillea spectabilis roots;purification and characterisation. Phytochemistry 1998;47(8):1561-5.

Grover IS, Bala S. Antimutagenic activity of Terminalia chebula (myroblan) in Salmonella typhimurium. Indian J Exp Biol 1992;30(4):339-41.

Published

01-07-2014

How to Cite

T., S., and A. R. “DETERMINATION OF PHYTOCOMPOUNDS FROM TERMINALIA CHEBULA RETZ BY HPTLC DENSITOMETRIC METHOD”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 516-20, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1753.

Issue

Section

Original Article(s)