ANTIOXIDANT CAPACITIES FROM DIFFERENT POLARITIES EXTRACTSOF THREE KINDS GINGERUSINGDPPH, FRAPASSAYS ANDCORRELATION WITH PHENOLIC, FLAVONOID, CAROTENOID CONTENT
Keywords:Antioxidants, FRAP, DPPH, Three kinds ginger, flavonoid, Phenolic, Carotenoid
Objectives: The objectives of this research were to study antioxidant capacityfrom different polarities extracts of three kinds ginger using two methods of antioxidant testing which were DPPH (2.2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total phenolic, flavonoid and carotenoid content in different polarities extracts of three kinds gingerwith DPPH and FRAP antioxidant capacities.
Methods: Extraction was performed by reflux using different polarities solvents. The extracts were vaporated using rota vapor. Then antioxidant capacities were tested using DPPH and FRAP assays. Determination of total phenolic, flavonoid and carotenoid content were performed by spectrophotometry UV-Vis and its correlation with FRAP and DPPH antioxidant capacities were analyzed by Pearson method.
Results: EG3(ethanol extract of elephant ginger rhizomes) had the highest DPPH scavenging capacity with IC50 0.26 ppmandEG3had the highest FRAP capacity also with EC5091.90 ppm.SG1(n-hexane extract of small ginger) contained the highest total phenolic (14.56 g GAE/100 g), EG2(ethyl acetate extract of elephant ginger rhizomes) had highest flavonoid content (7.5 gQE/100 g) andEG3 had the highest carotenoid 0.95 g BET/100 g.
Conclusions: There were positively high correlation between total phenolic content in small ginger rhizomes extracts with their antioxidant activity using DPPH and FRAP assays. DPPH scavenging capacities in small ginger rhizomes extracts had positively high correlation with their FRAP capacities.
Jain N, Goyal S, J. Ramawat KGEvaluation of antioxidant properties and total phenolic content of medicinal plants used in diet therapy during postpartum healthcare in Rajasthan. Int PharmSci 2011;3(3):248-53.
Thaipong K, Boonprakob U, Crosby K, Zevallos LC, J. Byrne DHComparison of FRAP, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Comp Anal 2006;19:669-75.
Ling LT, D. U, Valdez B. Palanisamy, Potential antioxidants from tropical plants, In:editor, Food industrial processes-methods, Kuala Lumpur:In Tech;. p1999. 64-72.
Souri E, Amin G, Farsan H, M. T, J. U. Barazandeh antioxidant activity and phenolic content of 24 medicinal plats extracts. DARSci 2008;16:83-7.
Luis A, Gil N, Amaral ME, P. A, J. Duarte activities of extract from Acacia melanoxylon, Acacia dealbata, Oleaeuropaeaand alkaloids estimation. Int PharmSci 2012;4:225-31.
Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe):a review of recent research. Food and chemical toxicology:an international journal published for the British Industrial Biological Research Association 2008;46(2):409-20.
Masuda Y, Kikuzaki H, Hisamoto M, Nakatani N. Antioxidant properties of gingerol related compounds from ginger. BioFactors (Oxford, England) 2004;21(1-4):293-6.
Tahtawy RHM, Bastawesy AM, Monem MGA, Zekry ZK, Mehdar HA, M. M, et al. Merzabani activity of the volatile oils of Zingiberofficinale (ginger). BioFactors (Oxford, England) 2011;1(1):1-8.
Huang W-y, Zhang H-c, Liu W-x, Li C-y. Survey of antioxidant capacity and phenolic composition of blueberry, blackberry, and strawberry in Nanjing. Journal of Zhejiang University Science B 2012;13(2):94-102.
Agbor GA, Moumbegna P, Oluwasola EO, Nwosu LU, Njoku R-CC, Kanu S, et al. Antioxidant capacity of some plant foods and beverages consumed in the Eastern Region of Nigeria. African journal of traditional, complementary, and alternative medicines:AJTCAM / African Networks on Ethnomedicines 2011;8(4):362-9.
S. M. Blois determination by the use of stable free radicals. Nature 1958;181:1199-2000.
Bedawey AA Characteristics of antioxidant isolated from some plants sources, Cairo:Shibin El-Kom;2010, p. 1-11.
Benzi IFF, Strain JJ The ferric reducing ability of plasma (FRAP) as a measure of antioxidant powerâ€:the FRAP assay. Anal Biochem 1996;239:70-76.
Pourmorad F, Hosseinimehr SJ, N. Shahabimajd activity, phenol and flavonoid content of some selected Iranian medicinal plants. AfrJBiotechnol 2006;5(11):1142-5.
Chang CC, Yang MH, Wen HM, C. J, J. Chern of total flavonoid content in propolis by two complementary colorimetric methods. Drug Anal 2002;10:178-82.
Li XC, Wang XZ, Chen DF, Chen SZ Antioxidant activity and mechanism of protochatechuic acid in vitro. J Funct Food Health Dis 2011;1:232-244.
Apak R, GÃ¼Ã§lÃ¼ K, Demirata B, OzyÃ¼rek M, Celik SE, BektaÅŸoÄŸlu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules (Basel, Switzerland) 2007;12(7):1496-547.
Stoilova TB, Dutseva EA, Pashkovskaia AA, Sychev SV, Koval'chuk SI, Sobko AA, et al. [Ion channels of various types induced in lipid membranes by gramicidin A derivatives carrying a cationic sequence at their C-termini]. Bioorg Khim 2007;33(5):511-9.
Maizura M, Aminah A, M. WW, Res J. Aida phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiberofficinale) and turmeric (Curcuma longa) extract. Int Food. BioFactors (Oxford, England) 2011;18:529-34.
Sattar NA, Hussain F, T., Bangladesh J. Iqbal activities of Z. officinaleRoscoe and A allughasRoscoe Zingiberaceae RhizomesSci Ind Res 2013;48(2):115-8.
Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules (Basel, Switzerland) 2010;15(6):4324-33.
Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants:chemistry, metabolism and structure-activity relationships. The Journal of nutritional biochemistry 2002;13(10):572-84.
Foote CS. Free radicals in biology1976.
Beutner S, Bloedorn B, Hoffmann T, Martin HD. Synthetic singlet oxygen quenchers. Methods Enzymol 2000;319:226-41.
Kobayashi H, Kitai R, Ido K, Kabuto M, Handa Y, Kubota T, et al. Hemodynamic and metabolic changes following cerebral revascularization in patients with cerebral occlusive diseases. Neurol Res 1999;21(2):153-60.
V. MÃ¼ller L, FrÃ¶hlich K, BÃ¶hm antioxidant activities of carotenoids measured by ferricreducing antioxidant power (FRAP), ABTS bleaching assay (aTEAC),DPPH assay and peroxyl radical scavenging assay. Food Chem 2011;129:139-48.
Charles DJ Antioxidant properties of spices herbs and other. London:John Willey;2013.
Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53(6):1841-56.