ISOLATION AND CHARACTERIZATION OF A NOVEL CHEMICAL COMPOUND FROM EUGENIA CARYOPHYLLUS FLOWER BUD EXTRACT
Keywords:
Eugenia caryophyllus, Myrtaceae, Flower buds, Gossypetin, Syzygium aromaticumAbstract
Objective: The objective of this study was to isolate and characterize the bioactive principles from the aqueous ethanolic extract of flower buds of Eugenia caryophyllus.
Methods: The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis.
Results: The infra-red spectra showed specific absorption bands for flavanoids viz. 1245.86 to 1111.01 cm-1 for HCO stretch of ether; 1330 to 1050 cm-1 for CO stretch of lactone. In addition IR spectrum showed specific absorption bands for flavanoids viz. 3432.57cm-1 for OH stretch for alcohol and phenol. Mass spectra showed pseudomolecular ion (M+ion) peak at m/z 464 which corresponds to molecular formula C21H20O12.
Conclusion: From the spectral characteristics, the isolated compound from the extract was confirmed to be Gossypetin 7-O rhamnopyranoside (Rhodiolgin).
Downloads
References
Sasidharan S, Chen Y, Saravanan D, Sundram KM, Latha LY. Extraction, isolation and Characterization of Bioactive compounds from Plant extracts. Afr J Tradit Complement Altern Med 2011;8(1):1-10.
WHO Report on Traditional Medicine WPR/RC 52/76th. Aug. 2001.
Chaieb K, Hajlaoui H, Zmantar T, Nakbi ABK, Rouabhia M, Mahdouani K, et al. The chemical composition and biological activity of essential oil, Eugenia cryophyllata (Syzygium aromaticum L. Myrtaceae):a short review. Phytother Res 2007;21(6):501-6.
Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu India., BMC Complement Altern Med 2006;6:35.
Fu Y, Zu Y, Chen L, Shi X, Wang Z, Sun S, et al. Antimicrobial activity of clove and rosemary essential oils alone and in combination. Phytother Res 2007;21:989–94.
Park MJ, Gwak KS, Yang I, Choi WS, Jo HJ, Chang WJ, et al. Antifungal activities of the essential oils in Syzygium aromaticum (L.) Merr. Et Perry and Leptospermum betersonni Bailey and their constituents against various dermatophytes. J Microbiology 2007;45:460-65.
Cai L, Wu CD. Compounds from Syzygium aromaticum possessing growth inhibitory activity against oral pathogens. J Nat Prod 1996;59(10):987-90.
Miyazawa M, Hisama M, Antimutagenic activity of phenylpropanoides from clove (Syzygium aromaticum). J Agric Food Chem 2003;51(22):6413-22.
Magalhaes CB, Riva DR, DePaula LJ, Brando-Lima A, Koatz VL, Leal-Cardoso JH, et al. In vivo anti-inflammatory action of eugenol on lipopolysaccharide-induced lung injury. J Appl Physiol (1985);108(4):845-51.
Li Y, Xu C, Zhang Q, Liu JY, Tan RX. In vitro anti-Helicobacter pylori action of 30 Chinese herbal medicines used to treat ulcer diseases. J Ethnopharmacol 2005;98(3):329-33.
Bae EA, Han MJ, Kim NJ, Kim DH. Anti-Helicobacter pylori activity of herbal medicines. Biol Pharm Bull 1998;21(9):990-92.
Srivastava KC, Malhotra N. Acetyl Eugenol, a component of oil of cloves (Syzygium aromaticum) inhibits aggregation and alters arachidonic acid metabolism in human blood platelets. Prostaglandins Leukot Essent Fatty Acids 1991;42:73-81.
Cosa P, Vlientinck AJ, Berghe DV, Maes L. Anti-infective potential of natural products: How to develop a stronger In vitro ‘proof-of-concept’. J Ethnopharmacol 2006;106:290-302.
Pourgholami MH, Kamalinejad M, Jayadi M, Majzoob S, Sayyah M. Evaluation of anti-convulsant activity of essential oil of Eugenia caryophyllata in male mice. J Ethnopharmacol 1999;64(2):167-71.
Kumaravelu P, Subramaniyam S, Dakshinamoorthy DP, Devaraj NS. The antioxidant effect of eugenol on CCl4-induced erythrocyte damage in rats. J of Nut Biochem 1996;7(1):23-8.
Singh AK, Dhamanigi SS, Asad M. Anti-stress activity of hydro-alcoholic extract of Eugenia caryophyllus buds (clove). Indian J Pharmacol 2009;41:28-31.
Hossseini M, Kamakar M,Rakhshandeh H. Analgesic effect of clove essential oil in mice. Avicenna J Phytomed 2011;1(1):1-6.
Kamkar M, Nazaribourun A, Hosseini M. Analgesic effect of aqueous and ethanolic extracts of clove. Avicenna J Phytomed 2013;3 (2):186-92.
Srivastava KC. Antiplatelet principles from a food spice clove (Syzgium aromaticum). Prostaglandins, Leukotrienes Essent. Fatty Acids 1993;48:363-72.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 41st ed. India:Nirali Prakashan;2008.
Harborne JB. Phytochemical Methods:A guide to modern techniques of plant analysis. 3rd ed. UK:Springer;1998.
Silverstein RM, Webster FX. Spectrometric identification of organic compounds. 6th ed. India:Wiley;2005