METHYLPHENIDATE: PROCONVULSANT EFFECT AND ACTION ON ACETYL CHOLINESTERASE ACTIVITY IN YOUNG AND ADULT MICE
Keywords:Acetylcholinesterase, Hippocampus, Methylphenidate, Seizures, Striatum
Objective: Methylphenidate (MPH) is a derivative of piperidine, structurally related to amphetamine. It is the most widely psychostimulant used in Brazil for treating attention deficit disorder and hyperactivity. This drug was investigated in an epilepsy model induced by pilocarpine and on acetylcholinesterase (AChE) activity in young and adult mice.
Methods: The control group was treated with saline. The treated groups received MPH (2.5, 5, 10 or 20 mg/kg) in single dose, followed by pilocarpine (400mg/Kg). The groups were observed for1h after treatment.
Results: MPH, in all doses, was efficacious in decreasing both the latency to first seizures and the survival percentage in young and adult animals. Determination of AChE activity in the hippocampus and striatum of young and adult animals, after pilocarpine-induced status epilepticus, demonstrated that pretreatment with MPH reduced AChE activity only in the striatum.
Conclusion: Our findings suggest that MPH has proconvulsant action and cholinergic neurotransmission system can play a role in this effect.
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