NEUROPROTECTIVE ACTIVITY OF FRACTIONAL FLOWER EXTRACTS OF MIRABILIS JALAPA AGAINST ALUMINIUM HYDROCHLORIDE INDUCED NEUROTOXICITY IN MALE WISTER RATS
DOI:
https://doi.org/10.22159/ijpps.2017v9i5.17584Keywords:
Mirabilis jalapa, Memantine, Oxidative stress, Thiobarbituric acid reactive substances, Neuroprotective activityAbstract
Objective: The major objective of this present study was to evaluate the neuroprotective effect of fractional flower extracts (acetone, petroleum ether, methanol and aqueous) of Mirabilis jalapa (MJ) against aluminium hydrochloride-induced neurotoxicity in male wister rats.
Methods: From the different fractional flower extracts of Mirabilis jalapa (MJ), two doses (250 and 500 mg/kg body weight) of each extract was initially selected and administered per orally 30 min prior to aluminium hydrochloride administration to the different animal groups once a day for a period of 45 d. Rat serum was collected from different animal groups on 1st, 15th, 30th and 45th days for estimation of marker enzymes, where a reduction in marker was observed. Animal was sacrificed by decapitation and the whole brain of rats was analyzed to estimate the levels of nitrite, thiobarbituric acid reactive substances (TBARS), superoxide dismutase (DOS), catalase, reduced glutathione and acetylcholinesterase (AchE).
Results: On the 9th day the Wister rats were sacrificed and cerebral cortex was removed. One-half of the cerebral cortex samples from different groups of Aluminium hydrochloride treated rats were stored in FAM mixture (40% formaldehyde, acetic acid and methanol in the ratio of 1:1:8) for histological analysis. From the study confirmed that dose of 250 and 500 mg/kg bwt of methanolic extract of MJ significantly (p˂0.001) increases the reduced glutathione, superoxide dismutase and catalase level, whereas petroleum ether, acetone and aqueous fractional flower extracts of MJ significantly (p˂0.01) decreases nitrite, TBARS and AchE levels of aluminium hydrochloride treated groups.
Conclusion: This result is indicating evidence for Mirabilis jalapa had a significant neuroprotective effect on aluminium hydrochloride-induced neurotoxicity and also supports by histopathological studies.
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