• Marimuthu Krishnaveni Assistant Professor, Department of Biochemistry, Periyar University, Salem- 636011


Antioxidant, Bauhinia purpurea, Dried leaves, Phenolics, Flavonoids


Objective: Bauhinia purpurea is called as Mandarai in Tamil and in English as butterfly tree. The aim of the present study was to determine the secondary metabolites and antioxidant activities of shade dried Bauhinia purpurea leaf aqueous extract.

Methods: Quantitative analysis for total phenolics was done by Folin-ciocalteau method and total flavonoids by aluminium chloride method. Likewise, various antioxidant activities were assessed by following standard methods.

Results: The flavonoid content was higher (160.0±6.9mg/g) compared to phenolics (126.66± 6.11mg/g). Similarly, the nitric oxide scavenging activity (258.66±4.61mg/g) and reducing power activity (141.33±2.30mg/g) was found to be higher compared to total antioxidant (81.33±6.11mg/g) and metal chelating activity (30.66±2.30mg/g).

Conclusion: The results obtained reveal that Bauhinia purpurea leaf extract proves to be a good antioxidant and needs further characterization to confirm its diversified therapeutic applications.


Download data is not yet available.


Rajanna LN, Sharanabasappa G, Seetharam YN, Aravind B and Mallikharjuna PB. In vitro Regeneration of Cotyledonary Node Explant of Bauhinia racemosa. Botany Research International. 2011;4(4):75-80.

Rajendra S and Madhukar B. Arboreal Flora of Solapur Corporation. Journal of Botanical Research. 2011;2(1):08-16.

Singh G. Plant Systematics-Theory & Practice 2 Edition. Oxford and IBH publishing Co. Pvt.Ltd:New Delhi, 2004;398-402.

Khare CP. Encyclopedia of Indian Medicinal Plant. Springer Verlag, New York, 2004;95-96.

Chopda MZ and Mahajan RT. Wound Healing Plants of Jalgaon District of Maharashtra state, India. Ethnobotanical Leaflets. 2009;13(4):1-32.

Zakaria ZA, Rofiee MS, Teh LK, Salleh MZ, Sulaiman MR and Somchit MN. Bauhinia purpurea leaves’ extracts exhibited in vitro antiproliferative and antioxidant activities.†African Journal of Biotechnology. 2011;10(1):65– 74.

Annegowda HV, Mordi MN, Ramanathan S, Hamdan MR and Mansor SM. Effect of extraction techniques on phenolic content, antioxidant and antimicrobial activity of Bauhinia purpurea:HPTLC determination of antioxidants.†Food Analytical Methods. 2012;5(2):226–233.

Negi BS, Dave BP and Agarwal YK. Evaluation of antimicrobial activity of Bauhinia purpurea leaves under in vitro conditions.†Indian Journal of Microbiology.2012;52(3):60–365.

Fang EF, Bah CSF, Wong JH, Pan WL, Chan YS, Ye XJ, Ng TB. A potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds:from purification to mechanism exploration.†Archives of Toxicology. 2012;86(2):293–304.

Zakaria ZA, Abdul Hisam EE, Rofiee MS, Norhafizah M, Somchit LK Tech and Salleh MZ. In vivo antiulcer activity of the aqueous extract of Bauhinia purpurea leaf.†Journal of Ethnopharmacology. 2011;137(2):1047– 1054.

Zakaria ZA, Abdul Hisam EE, Norhafizah M, Rofiee MS, Othman F,Hasiah AH and Vasudevan M. Methanol extract of Bauhinia purpurea leaf possesses Anti-Ulcer Activity.†Medical Principles and Practice. 2012;21(5):476–482.

Nascimento GGF, Lacatelli J, Freitas PC and Silva GL. Antibacterial activity of plants extracts and phytochemical on antibiotic resistant bacteria. Braz J Microbiol. 2000;31(4):886 – 891.

Ebrahimzadeh MA, Hosseinimehr SJ, Hamidinia A and Jafari M. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology online. 2008;1:7-14.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Hamidinia A, and Bekhradni AR, Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica, Pharmacology online. 2008;2:560-567.

Mervat MM, Far E, Hanan A and Taie A. Antioxidant activities, total anthrocynins, phenolics and flavonoids contents of some sweet potato genotypes under stress of different concentration of sucrose and sorbitol. Australian J Basic Applied Sci. 2009;3(4):3609-3616.

Prieto P, Pineda M, and Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex:specific application to the determination of Vitamin E. Anal Biochem. 1999;269(2):337-341.

Ebrahimzadeh MA, Nabavi SF and Nabavi SM. Antioxidant activities of methanol extract of Sambus ebulus L flower. Pak J Biol Sci. 2009;12(5):447-450.

Yen GC and Chen HY. Antioxidant activity of various tea extracts in relation to their Antimutagenicity. J Agri Food Chem. 1995;43(1):27-32.

Ebrahimzadeh MA, Pourmorad F and Bekhradnia AR. Iron chelating activity screening, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol, 2008a ;32: 43-49.

Forkmann G and Martens S. Metabolic engineering and applications of flavonoids. Current opinion in Biotechnology. 2001;12(2):155-160.

Weisshar B and Jenkins G. Phenylpropanoid biosynthesis and its regulation. Current Opinion plant Biology. 1998;1(3):251-257.

Winkel-Shirley B. Flavonoid Biosynthesis:A colourful model for Genetics, Biochemistry, cell biology and Biotechnology. Plant Physiology. 2001;126(2):485-493.

David S.Studies force new view on biology of flavonoidsâ€. EurekAlert. Oregon State University. URL, 2007.

Kalpna R, Mital K and Sumitra C. Vegetable and fruit peels as a novel source of antioxidants. J Med Plants Res. 2011;5(1):63-71.

Odabasoglu F, Aslan A, Cakir A, Suleyman H and Karagoz Y. Comparison of antioxidant activity and phenolic content of three lichen species. Phytother Res. 2004;18(11):938-941.

Sini K, Sinha B and Karpagavalli M. Determining the antioxidant activity of certain medicinal plants of Attapady, (Palakkad), India using DPPH assay. Curr Bot J. 2010;1(1):13-17.

Mahoba B Nalli, Rabia O Alghazeer, Nabil A Saleh and Asma Y Al-Najjar. Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea). Arab J Chem. 2010;3(2):79-84.

Sulaiman S, Ibrahim D, Kassim J and Sheh Hong L. Antimicrobial and antioxidant activities of condensed tannin from Rhizophora apiculata barks. J Chem Pharm Res. 2011;3(4):436-444.



How to Cite

Krishnaveni, M. “ANTIOXIDANT POTENTIAL OF BAUHINIA PURPUREA (L) LEAF”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 558-60,



Original Article(s)