ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF GYNANDROPSIS PENTAPHYLLA LINN ON MCF-7 CELL LINE

Authors

  • Susithra Rajendran Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.
  • Renuka Saravanan Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.
  • Sivakumar Ramalingam Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.
  • Sheik Abdulla Shahul Hameed Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujam Centre, Kumbakonam 612001, Tamil Nadu, India.

Keywords:

Gynandropsis pentaphylla Linn, Antiproliferative, Antioxidant, Anticancer

Abstract

Objective: To investigate the in vitro Antiproliferative and Antioxidant Activity of Gynandropsis pentaphylla Linn. Leaves on MCF-7 Cell line.

Methods: Phytochemicals were analysed by using standard methods. In Vitro antioxidant studies were carried out for the methanol extracts of the Gynandropsis pentaphylla using various free radical models such a DPPH, Reducing power assay and Hydrogen peroxide. In vitro cytotoxic assay such as trypan blue dye exclusion and MTT assays were carried out in methanolic extract against MCF-7 cell line.

Results: The results of the present study demonstrated that the qualitative phytochemical analysis of the methanol, aqueous and chloroform extract of Gynandropsis pentaphylla showed the presence of carbohydrates, flavonoids, tannins, glycosides, phenols, saponins, protein and terpenoids. The antioxidant activity of methanolic extract of leaves G.pentaphyllawas confirmed by free radical scavenging activity and reducing (FRAP) and it was found to be significant. Inhibitory concentration (IC50) for the methanolic extract of Gynandropsis pentaphylla on MCF-7 cell was found to be 96.67 determined by MTT assay.

Conclusion: It revealed that the crude methanolic extract of G. pentaphylla has antiproliferative activity against MCF-7 cell lines. From our study it is concluded that, the phytochemicals present in the Gynandropsis pentaphylla possess antioxidant and anticancer activity.

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Published

01-07-2014

How to Cite

Rajendran, S., R. Saravanan, S. Ramalingam, and S. A. S. Hameed. “ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF GYNANDROPSIS PENTAPHYLLA LINN ON MCF-7 CELL LINE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 561-5, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1761.

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