GASTRO PROTECTIVE AND ANTI-HELICOBACTER PYLORI EFFECTS OF A FLAVONOID RICH FRACTION OBTAINED FROM ACHYROCLINE SATUREOIDES (LAM) D.C.

Authors

  • JOSE ROBERTO SANTIN Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • Marivane Lemos Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • LUIZ CARLOS KLEIN-JUNIOR Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • Alessandro Conrado D. E. Oliveira Silveira Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil, Curso de Farmacia, RuaAntônio da Veiga, 140, Universidadede Blumenau-FURB, Blumenau, Santa Catarina, Brazil
  • Marcel Petreanu Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • Tailyn Zermiani Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • Alexandre Bella Cruz Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • Rivaldo Niero Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil
  • Sergio Faloni D. E. Andrade Programa de Pos-graduacaoem Ciencias Farmaceuticas, Nucleo de Investigacoes Quimico-Farmaceuticas (NIQFAR), Rua Uruguai, 458, Universidade do Vale do Itajai–UNIVALI, Itajai, Santa Catarina, Brazil

Keywords:

Achyrocline satureoides, Gastroprotective, Helicobacter pylori, Flavonoids

Abstract

Objectives: The study aimed to investigate the gastroprotective effects of a flavonoid rich fraction (FRF) obtained from Achyrocline satureoides.

Methods: The following protocols were employed: ethanol and NSAID-induced ulcer, ligature pylorus model, and free mucus quantification. Nitric oxide (NO) and sulfhydryl group participation were observed by pretreatment with L-NAME or NEM. Besides, it was assayed the acetic acid-induced chronic ulcer andthe anti-Helicobacter pyloriactivity in vitro.

Results: The phytochemical profile of FRF showed three main flavonoids, luteolin, quercetin and 3-O-methyl-quercetin. The administration of FRF was able to prevent the damage evoked by ethanol and NSAID-induced ulcer models. The pH and concentration of H+ in the stomach were not modified by FRF treatment. However, the FRF treatment induces mucus secretion. The effect presented by FRF was mediated by nitric oxide (NO). In chronic ulcer model FRF reduced significantly the lesion area, promoting a cure ratio of 65.42±13.00, a similar data presented by cimetidine treated animals (61.35±11.88). Using an in vitro assay was observed that FRF at 500 µg/mL was able to inhibit bacterial growth.

Conclusions: The results show that FRF provided a significant gastroprotective and ulcer healing activity, mainly due to their capacity to enhance mucus secretion.

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Published

01-07-2014

How to Cite

SANTIN, J. R., M. Lemos, L. C. KLEIN-JUNIOR, A. C. D. E. O. Silveira, M. Petreanu, T. Zermiani, A. B. Cruz, R. Niero, and S. F. D. E. Andrade. “C”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 417-22, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1776.

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