COMPARATIVE ANALGESIC STUDIES OF LEAF AND STEM BARK OF CALOPHYLLUM INOPHYLLUM IN SWISS ALBINO MICE
Keywords:Calophyllum inophyllum, Calophyllum inophyllum leave extract, Calophyllum inophyllum stem bark extract, Analgesic effect, Eddy`s hotplate method, Acetic acid induced writhing
Objectives: The aim of the present study was to perform acute oral toxicity studies and to compare and evaluate the analgesic effect of ethanolic extracts of leaf and stem bark of Calophyllum inophyllum on Swiss albino mice.
Methods: Eddy's hotplate method and acetic acid induced writhing were implemented to determine analgesic properties of the extracts. Both the extracts were administered orally. Acute oral toxicity studies were conducted using the OECD guidelines 423 Annexure â€“ 2d.
Results: The results indicate the mortality was not observed during the acute oral toxicity studies and maximum safe does was determined. The analgesic effect of the extracts showed significant dose dependent effect (100 mg/kg b.w and 200 mg/kg b.w) on both models of algesia i.e., Eddy's hotplate method and acetic acid induced writhing.
Conclusion: The comparative studies between the leaf and stem bark of Calophyllum inophyllum suggests that Calophyllum inophyllum leaf extract showed more activity compared to Calophyllum inophyllum stem bark extract.
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