• Senthil Rani Soundharajan Department of Biotechnology, Anna University, Regional Centre, Coimbatore 641047, Tamil Nadu, India
  • Renuka Devi Ponnusamy Department of Biotechnology, Anna University, Regional Centre, Coimbatore 641047, Tamil Nadu, India


n-hexane, Ethyl acetate, Flavonoid, FT-IR, Phenol


Objectives: The present study, aimed to determine and characterize the chemical constituents of Cassia auriculata flower extract by qualitative, quantitative and analytical techniques.

Methods: Preliminary Phytochemical, total flavonoid and phenol content was determined in the methanolic extract of C.auriculata (CAFMEt) using standard methods. C-18 silica gel based column chromatography was used to purify CAFMEt using n-hexane, ethyl acetate and methanol and fraction identified by thin layer chromatography. GC-MS and FT-IR techniques used to characterize the lead fraction.

Results: CAFMEt showed the presence of flavonoid and Phenols in a significant amount. Three fractions was collected from column chromatography viz., Fraction 1-3 (n-hexane: yellow) was 2.5mg, (ethyl acetate: light orange) 1.8mg and (methanol: light green) 5.67mg respectively. TLC indicated n-hexane has higher refractive factors 0.457 at yellow band and ethyl acetate fraction has 0.329 at light orange band. 14 chemical constituents were identified by GC-MS included alkanes, alcohol, esters and hydrocarbons. The major peak showed the presence of 4-(4-methylphenoxy) phenol at 22.53%. Infra red spectra revealed the presence of phenolic groups in hexane fraction.

Conclusion: Further studies will be carried out the pharmacological potential of n-hexane fractions of flowers of C.auriculata.


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How to Cite

Soundharajan, S. R., and R. D. Ponnusamy. “CHEMICAL COMPOSITION AND CHARACTERIZATION STUDIES OF CASSIA AURICULATA FLOWER EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 327-30,



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