DETERMINATION OF TRAZODONE IN HUMAN PLASMA BY REVERSED-PHASE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY WITH ELECTROSPRAY IONISATION

Authors

  • Prashant Kale Gujarat Forensic Sciences University. Gandhinagar, India.
  • Y. K. Agrawal Gujarat Forensic Sciences University. Gandhinagar, India.
  • Shailendra Gupta Gujarat Forensic Sciences University. Gandhinagar, India.
  • Chirag Patel Gujarat Forensic Sciences University. Gandhinagar, India.
  • Ilesh Patel Gujarat Forensic Sciences University. Gandhinagar, India.

Keywords:

Trazodone, Trazodone d6, LC-MSMS

Abstract

Objective: The development and validation of LC-MS/MS method for quantification of Trazodone (a serotonin antagonist and reuptake inhibitor (SARI), which is a second generation antidepressant compound belonging to the class of phenyl piperazine) in human plasma is described.

Methods: The method involves protein precipitation (extraction) using Trazodone d6 as an internal standard (IS). Chromatographic separation is achieved on Zorbax eclipse XDB C8 150×4.6 mm, 5 μm column with a mobile phase consisting of 2 mM ammonium formate (pH 3.0) and methanol (30:70 v/v) at a flow rate of 1.0 mL / min and the total run time was 2.5 minute. Detection was carried out by AB Sciex API 3200 tandem mass spectrometer using positive electro-spray ionization mode by multiple reactions monitoring method at m/z 372.00→176.10 and 378.20→182.10 for Trazodone and Trazodone d6 (ISTD) respectively with dwell times of 300 msecs for each of the transitions.

Results: The standard curve was linear from 5.203 ng / mL to 3025.166 ng / mL with goodness of fit (r2) greater than 0.990 observed during the method validation batches. This assay allows quantification of Trazodone at a concentration as low as 5 ng / mL in human plasma. The observed mean recovery was 88% for the drug.

Conclusions: The method described here is found to be simple, cost effective and suitable for the use in bioequivalence and bioavailability studies.

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References

Bo Wen, Li Ma, A. David Rodrigues, and Mingshe Zhu. Detection of novel reactive metabolites of Trazodone:evidence for CYP2D6-Mediated bioactivation of m-Chlorophenylpiperazine. Drug Metab Dispos 2008;36(5):841-50.

Patel BN, Sharma N, Sanyal M, Shrivastav PS. High throughput and sensitive determination of trazodone and its primary metabolite, m-chlorophenylpiperazine, in human plasma by liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2008;871(1):44-54.

R. E. GAMMANS, A. V. MACKENTHUN & J. W. RUSSELL. Comparative bioavailability of trazodone formulations using stable isotope methodology Br. J Clin Pharmac 1984;18:431-7.

Published

01-07-2014

How to Cite

Kale, P., Y. K. Agrawal, S. Gupta, C. Patel, and I. Patel. “DETERMINATION OF TRAZODONE IN HUMAN PLASMA BY REVERSED-PHASE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY WITH ELECTROSPRAY IONISATION”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 300-4, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/1788.

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