PHYSICOCHEMICAL, PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY AGAINST CLINICAL PATHOGENS OF MEDICINALLY IMPORTANT ORCHID GEODORUM DENSIFLORUM (LAM) SCHLTR

Authors

  • M. Keerthiga Department of Biotechnology, National College (Autonomous), Tiruchirappalli–01
  • S. P. Anand Department of Botany, National College (Autonomous), Tiruchirappalli-01

Keywords:

G densiflorum, antibacterial, Phytochemical analysis, Physicochemical analysis

Abstract

Objective: To evaluate the Physicochemical, Preliminary Phytochemical analysis and Antibacterial activity of Geodorum densiflorum against clinical pathogens and to prove the orchid will target to meet the therapeutic demands.

Methods: The physicochemical parameters and Preliminary Phytochemical analysis of the orchid were studied. The antibacterial properties using the following bacterial strains, ie., Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes, Klebseilla pneumoniea, Pseudomonas aeruginosa were studied. Streptomycin were used as a control and study was conducted using disc diffusion method and the MIC value also be evaluated.

Results: The Physicochemical parameters show the presence of bioactive compounds. The phytochemical screening revealed the presence of Alkaloids, Tannins, Flavonoids, Sopanins, Steroid and Terpenoids. The ethanol extract showed significant total activity against Staphylococcus aureus than other extracts.

Conclusion: The ethanol extract of Geodorum densiflorum can successfully used as a pharmaceutical agents as antibacterial drug.

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References

Gnanamani A, Shanmuga Priya K, Radhakrishna N, Mary Babu. Antibacterial activity of two plant extracts on eight burn pathogens. J Ethanopharmacology 2003;86:59-61.

Evans W.c, Trease and Evans. Pharmacognosy. 14th edition. WB Sacender Company Ltd;1996.290.

Manoharan Sivananthan. Antibacterial activity of 50 medicinal plants used in folk medicine. Int J of Biosciences 2013;3(4):104-21.

Edeoga I HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. J Afr Biotech 2005;4:685-8.

Griesbach RJ. Orchids emerge as major world floral crop. J Chron Horticult 2003;43:6-12.

Cronquist A. An integrated system of classification of flowering plants. Columbia University Press:Ithaca;1981.

Datta KB, Kanjilal B, Desarker D. Artificial seed technology:Development of protocol in Geodorum densiflorum (Lam) Schltr. An endangered orchid. J Current Sci 1999;76:1142–45.

Ch Sudhakor Reddy, C Pattanaik, MSR Murthy, KN Reddy. EPTRI–ENVIS Newsletter:Hyderabad India;2005.11(4).

Dash PK, Sahoo S, Bal S. Ethnobotanical Studies on Orchids of Niyamgiri Hill Ranges Orissa, India. J Ethnobotanical Leaflets 2008;12:70-8.

Chowdhery HJ. Orchid Flora of Arunachal Pradesh. Bishen Singh Mahendra Pal Singh. Dehra Dun,1998.

Patil MV, Patil DA. Ethnomedicinal practices of nasik district, Maharashtra. Indian J of Traditional Knowledge 2005;4(3):287-90.

Nath M, Dutta BK, Hajara PK. Medicinal plants in major diseases by Dimasa tribes of Barak valley. Assam University J of Sci and Technology:Biological and Environmental Sci 2011;7:18-26.

Harborne JB. Phytochemical methods:a guide to modern techniques of plant analysis. 2nd edn. Chapman and Hall: London;1994.p.1-35.

Khandelwal KR. Practical Pharmacognosy Techniques and Experiments. 15th ed. Pune:Nirali Prakashan;2006.p.15–163.

Brain KR, Turner TD. A Practical Evaluation of Phytopharmaceuticals, Bristol:Wright Scientechnica;1975.

Kokate CK. Plant Constituents In:Practical Pharmacognosy. 1st ed. Delhi:Vallabh Prakashan;1986.p.111-5.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken HR Manual of Clinical Microbiology, 6th Ed. ASM Press:Washington DC;1995:15-8.

Olurinola PF. A laboratory manual of pharmaceutical microbiology. Idu, Abuja, Nigeria, 1996;69-105.

Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA. Screening of crude extracts of six medicinal plants used in South–West Nigerian unorthodox medicine for antimethicillin resistant S. aureus activity. J BMC Comp Alt Med 2005;5(6):1-7.

Periyasamy Ashokkumar, Rajkumar and Mahalingam Kanimozhi. Phytochemical Screening and Antimicrobial Actiivity from Five Indian Medicinal Plants against Human Pathogens. Middle-East J of Scientific Res 2010;5(6):477-82.

Ammara Hassan, salma Rahman, Farah Deepa, Shahid Mahmud. Antimicrobial activity of some plant extracts having hepatoprotective effects. J of Medicinal Plants Res 2009;3(1):20-3.

Eloff JN. Quantifying the bioactivity of the plant extracts during screening and bioassay–guided fractionation. J Phytomedicine 2004;11(4):370-1.

Jain T, Sharma K. Assay of antibacterial activity of Polyalthia longifolia (Benth & Hook) leaf extracts. J of Cell and Tissue Res 2009;9(2):1817-20.

Pravin Aabsing Theng and AN Korpenwar. Studies on Phytochemical Pharmacognostic and Physicochemical investigations of an Endangered Orchid-Geodorum densiflorum (Lam.) Schltr. Inte J of Bioassay 2014;3(2):1771-4.

Published

31-08-2014

How to Cite

Keerthiga, M., and S. P. Anand. “PHYSICOCHEMICAL, PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY AGAINST CLINICAL PATHOGENS OF MEDICINALLY IMPORTANT ORCHID GEODORUM DENSIFLORUM (LAM) SCHLTR”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 558-61, https://journals.innovareacademics.in/index.php/ijpps/article/view/1925.

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