EFFECT OF COSTUS PICTUS D. DON ON CARBOHYDRATE HYDROLYZING ENZYMES

Authors

  • Anuya Rege Department of Virology & Immunology, Haffkine Institute, Parel, Mumbai 400012, India.
  • Ramkrishna Ambaye Department of Virology & Immunology, Haffkine Institute, Parel, Mumbai 400012, India.
  • Abhay Chowdhary Department of Virology & Immunology, Haffkine Institute, Parel, Mumbai 400012, India.

Keywords:

Alpha-amylase, Alpha-glucosidase, Costus pictus, Antidiabetic

Abstract

Objective: Costus pictus D. Don is a potent anti-diabetic plant used in folk as well as Indian traditional medicine. In the present study, 4 extracts namely, Fresh-aqueous, Fresh-hydroalcoholic, Dry-Aqueous and Dry-hydroalcoholic prepared from fresh as well as dried leaves of C. pictus were evaluated for their effect on alpha-amylase and alpha-glucosidase enzymes using In vitro assays.

Methods: Alpha-Amylase inhibitory activity of C. pictus extracts was evaluated using Porcine pancreatic alpha-amylase (PPA) with starch as a substrate, whereas, alpha-glucosidase inhibitory activity was evaluated using p-nitrophenyl-α-D-Glucopyranoside (PNPG) as a substrate.

Results: Only Fresh-aqueous extract of C. pictus revealed potent alpha-amylase inhibitory (IC50= 9.90mg/ml) as well as alpha-glucosidase inhibitory (IC50= 2.51mg/ml) activities. In general, it exhibited strong inhibitory effect on alpha-glucosidase than alpha amylase.

Conclusion: Present study does seem to justify usage of fresh leaves of C. pictus as a munching dilatory supplement for treatment of diabetes as Fresh-aqueous extract exhibited potent inhibitory effect on carbohydrate hydrolyzing enzymes.

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References

Narkhede MB, Ajimire PV, Wagh AE, Mohan M, Shivashanmugam AT, J. In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. J Asian Sci Res 2011;1:101-6.

Okoli CO, Obidike IC, Ezike AC, Akah PA, Salawu OA. Studies on the possible mechanisms of antidiabetic activity of extract of aerial parts of Phyllanthus niruri. J Pharm Biol 2011;49(3):248-55.

Jyothi KSN, Hemalatha P, Challa S, J. Evaluation of α-amylase inhibitory potential of three medicinally important traditional wild food plants of India. J Int Pharm 2011;5:95-9.

Vishwanathan BS, Darshan MV, Shekar MA. Prevention of chronic complications of diabetes mellitus-Does patient education score over treatment?. J Curr Sci 2002;83:1435-6.

Chakrabarti R, Rajagopalan R. Diabetes and insulin resistance associated disorders:disease and the therapy. J Curr Sci 83.2002:1533-8.

Punyarani K, Sharma JG. Micropropagation and microrhizome induction in Costus pictus D. Don using In vitro and ex vitro nodal segments as explant. J Not Sci Biol 2012;4:72-8.

Jayasri MA, Gunasekaran S, Radha A, Mathew TL, J. Anti-diabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. J Int Metab 2008;16:117-22.

Sudha P, Zinjarde SS, Bhargava S, Kumar AR, C. BM. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. J Altern Med 2011;11(5).

Bachhawat A, Shihabudeen MS, Thirumurugan K, J. Screening of fifteen Indian Ayurvedic plants for α-glucosidase inhibitory activity and enzyme kinetics. J Int Pharm Sci 2011;3:267-74.

Mohan V, Sandeep S, Deepa R, Shah B, Varghese C. Epidemiology of type 2 diabetes:indian scenario. Indian J of Medical Res 2007;125(3):217-30.

Ramachandran A, Snehalatha C, Viswanathan V. Burden of type 2 diabetes and its complications-The Indian Scenario. J Curr Sci 2002;83:1471-6.

Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M. Herbal antioxidant in clinical practice:a review. Asian Pacific J of Tropical Biomedicine 2014;4(1):78-84.

Kumar S, Kumar V, Rana M, Kumar D. Enzyme inhibitors from plants:an alternate approach to treat diabetes. J Pharmacogn Commun 2012;2:18-33.

Tripathi AK, Bhoyar PK, Baheti JR, Biyani DM, Khalique M, Kothmire MS, et al. Herbal antidiabetics:a review. J Int Pharm Sci 2011;2:30-7.

Ani V, Naidu KA. Antihyperglycemic activity of polyphenolic components of black/bitter cumin Centratherum anthelminticum (L.) Kuntze seeds. J Eur Food Res Technol 2008;226:897-903.

Giacco F, Brownlee M. Oxidative stress and diabetic complications. J Circ Res 2010;107(9):1058-70.

Suganya S, Narmadha R, Gopalakrishnan VK, Devaki K, J. Hypoglycemic effect of Costus pictus D. Don on alloxan induced type 2 diabetes mellitus in albino rats. J Asian Pac Dis 2012;2:117-23.

Shiny TC, Saxena A, Gupta SP. Phytochemical and hypoglycemic activity of Costus pictus plants from Kerala and Tamilnadu. Int J Pharm Sci Invent 2013;2:11-18.

Sindhi V, Gupta V, Sharma K, Bhatnagar S, Kumari R, Dhaka N, et al. Potential applications of antioxidants-a review. J Res 2013;7:828-35.

Hara Y, Honda M. The inhibition of α-amylase by tea polyphenols. J Agric Biol Chem 1990;54:1939-45.

Toda M, Kawabata J, Kasai T. Alpha-glucosidase inhibitors from clove (Syzgium aromaticum). J Biosci Biotechnol Biochem 2000;64(2):294-8.

Published

01-07-2014

How to Cite

Rege, A., R. Ambaye, and A. Chowdhary. “EFFECT OF COSTUS PICTUS D. DON ON CARBOHYDRATE HYDROLYZING ENZYMES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 278-80, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2006.

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