PHYSICOCHEMICAL, PHYTOCHEMICAL AND ANTIMICROBIAL PROPERTIES OF WILD HONEY COLLECTED AT MANGROVE AND MOUNTAIN AREAS IN SABAH, MALAYSIAN BORNEO

Authors

  • Philip Yap Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah (UMS), Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
  • Mohd Fadzelly Abu Bakar Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah (UMS), Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia, Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia (UTHM), 86400 Parit Raja, Batu Pahat, Johor, Malaysia

Keywords:

Wild honey, Physicochemical, Phytochemicals, Antimicrobials

Abstract

Objective: The aim of this study was to determine the physicochemical, phytochemical content and antimicrobial properties of selected honey of Sabah, Malaysian Borneo.

Methods: A standardized protocols were used to evaluate the physicochemical properties of selected honey of Sabah while the phytochemicals content (phenolics and flavonoids) were determined using Folin-Ciocalteau and aluminium colorimetric methods. Antimicrobial properties were evaluated using disc diffusion assay.

Results: For 80% methanol extract, old Upper Mountain honey contained the highest free acidity, conductivity, total phenolic and flavonoid contents with the values 23.84 ± 0.42 ml/g, 0.61 ± 0.01 mS/cm, 9.71 ± 0.01 mg gallic acid equivalent (GAE)/g and 7.76 ± 0.02 mg rutin equivalent (RU)/g, respectively. Antimicrobial activity showed strong inhibition by old Upper Mountain honey extract (80% methanol extract) with the value of 6.00 ± 0.01 mm at concentration of 100% against gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis). The same trend of phytochemicals content and antimicrobial activity was also observed in absolute methanol extracts.

Conclusion: The present results suggested that wild raw honey collected at mangrove and mountain area in Sabah contained a wide range of phytochemical compounds which has the potential for human health.

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References

Sanaa E-TK, Sanaa YO. Compression study of anti-microbial activity of honey-bees. Res J Microbiol 2007;2 (10):776-81.

Jasna B, Urska D, Mojca J, Terezija G. Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. J Food Chem 2007;105(10):822-28.

Mohapatra DP, Thankur V, Brar SK. Antibacterial efficacy of raw and processed honey. Int J of Biotechnol Res 2010;2011(Article ID 917505):6 pages

Alvarez-Suarez JM, Tulipani S, Romandini S, Vidal A, Battino M. Methodological aspects about determination of phenolic compounds and in vitro evaluation of antioxidant capacity in honey:A Review. J Curr Anal Chem 2005;5 (4):293-302.

Cooper RA, Molan PC, Molan, Harding KG. The sensitivity to honey of gram-positive cocci of clinical significance isolated from wounds. J Appl Microbiol 2002;5(93):857-63.

Radwan SS, El-Essawy AA, Sarhan MM. Experimental evidence for the occurrence in honey of specific substances active against microorganisms. Braz J Microbiol 2008;39(1):40-43.

Suhail M, Faizul-Suhail M. Oxidant-antioxidant status in pair matched maternal and cord blood of normotensive and preeclamptic patients. J Chin Clin Med 2009;4(5):241-8.

Ali TA, Chowdhury MN, Al-Humayyd MS. Inhibitory effect of natural honey on Helicobacter pylori. J Appl Microbiol 1991;4 (12):139-143.

Oyeleke BS, Dauda NEB, Jimoh T, Musa OS. Nutritional analysis and antibacterial effect of honey on bacterial wound pathogens. J Appl Sci 2010;11(6):1561-1565.

Hassanain TA, Alyaa KA, Karim JA. Antimicrobial effect of Malaysian honey on some human pathogens:an in vitro study. Ind J Med Res 2010;2(9):98-103.

Dimitrova B, Gevrenova R, Anklam E. Analysis of phenolic acids in honey of different floral origin by solid-phase extraction and high performance liquid chromatography. J Phyto Anal 2007;1(18):24-32.

Braide W, Oranusi SU, Akaluka CK, Nwaoguikpe RN, Akobundu CI, Peter NI. Antibacterial efficacy of crude and diluted honey on four wound isolates. J Microbiol 2012;1(1):1-4.

Zhao W, Li JJ, Yue SQ, Zhang LY, Dou KF. Antioxidant activity and hepatoprotective effect of a polyssacharide from Bei Chaichu (Bupleurum Chinense DC). J Carbohyd Polym 2012;89(2):448-52.

Aljadi AM, Kamaruddin MY. Evaluation of the total phenolic contents and antioxidant capacities of two Malaysian floral honeys. J Food Chem 2004;85:513-18.

Ahmed, M, Djebli N, Si MH, Meslem A, Aisaat S. Antibacterial activity of various honey types of Algeria against Staphyloccocus aureus and staphylococcus pyogenes. Asian Pac J Trop Med 2012;4:773-6.

Mervat MA, El-Gendy. In vitro evaluation of medicinal activity of Egyptian honey from different floral sources as anticancer and antimycotic infective agents. J Microbial Biochem Technol 2010;5(2):1-6.

Bogdanov S, Martin P, Lullmann C, Borneck R, Flamini CH, Morlot M et al. Harmonised methods of the European Honey Commission. J Apidologie 1997;2(11):1-59.

Bogdanov S, Martin P, Lullmann C, Borneck R, Flamini CH, Morlot M et al.. Honey quality, methods of analysis and international regulatory standards:review of the work of the International Honey Commission. J Apidologie 1999;4(14):108-25.

Beretta G, Granata P, Ferrero M, Orioli M, Facino MR. Standardization of antioxidant property of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. J Anal Chim Act 2005;26(33):135-91.

Meda A, Lamien CE, Romito M, Millogo J, Nacouma OG. Determination of the total phenolic, flavonoid and proline contents in Burkisa Fasan honey, as well as their radical scavenging activity. J Food Chem 2005;1(4):571-77.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. J Meth Enzymol 1999;21(84):152-78.

Zhishen J, Mencheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. J Food Chem 1999;64 (24):555-9.

Asli O, Bruce D, Kadriye S.Total phenolic acid and total flavonoid content of Turkish pine honeydew honey. JAAS 2010;2(4):65-71.

Jianguo C, Xian X, Xiling D, Jianbo X, Quanxi W, Andrae-Marobela K et al. Flavonoids profiles, antioxidants, acetylcholinesterase inhibition activities of extract Dyoathyrium boryanum (Willd.) Ching. J Food Chem Toxicol 2013;2(10):121-8.

Burt E. Antimicrobial Activities and Revelation to Environments. New York:The Uno Publisher. 2004. p.234-38.

Braide W, Oranusi SU, Akaluka CK, Nwaoguikpe RN, Akobundu CI, Peter NI. Antibacterial efficacy of crude and diluted honey on four wound isolates. J Microbiol 2012;1(3):1-4.

Lee CS, Norul-Liza AR, Mohamad SR, Ramlan A. Multi-elimental composition and physical properties of honey samples from Malaysia. J Food Chem 2012;12(135):880-7.

Terrab A, Recamales AF, Hernanz D, Heredia FJ. Characterization of Spanish thyme honey by their physiochemical characteristics and mineral contents. J Food Chem 2004;4(88):537-42.

Nuriza T, Arsyiah HAN, Shahjahan M, Noor Izani NJ, Munavvar SA, Absul KH, Mohsin SSJ. Antibacterial activity of local Malaysian honey. Malay J Pharm Sci 2005;3(2):1-10.

Georgil A, Korting C. Antifungal susceptibility testing with dermatofit. J Mycoses 1991;1(34):193-99.

Badet C, Quero F.The in vitro effect of Manuka honeys on growth and adherence of oral bacteria. J Anaerobe 2010;1(17):19-22.

Susana G, Luis DG, Leandro ML, Paula R, Leticia E. Physiochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. J Food Chem Toxicol 2009;1(48):544-8.

Amina C, Abderrahmane R, Gian ML, Paola F. Physiochemical properties of some honeys produced from different plants in Morocco. Arab J Chem 2011;1(1):1-9.

Tahany AH, Ghada SH, Amal AM. Isolation of antimicrobial peptides from Apis florae and Apis carnica in Saudi Arabia and investigation of the antimicrobial properties of natural honey samples. J King Saud Univ 2012;2(24):193-200.

Roula AM, Elias A, Elias B, Ziad D. Antibacterial activity of the extracts obtained from Rosmarinus officinalis, Origanum majorana, and Trigonella foenum-graecum on highly drug resistant gram Negative bacilli. J Bot 2010;1(Article ID.464087):1-8.

Agbaje EO, Ogunsanya T, Aiwerioba OIR. Conventional use of honey as antibacterial agent. J Ann Afr Med 2006;23(5):79-81.

Cooper RA, Molan PC, Harding KG. Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. JRSM 1999;10(92):283-5.

Mundo MA, Padilla-Zakour OI, Worobo RW. Growth inhibition of food borne pathogens and food spoilage organisms by select raw honey. Int J Food Microbiol 2004;12(97):1-8.

Basualdo C, Sgroy V, Finola MS, Marioli JM. Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds. J Vet Microbiol 2007;124(4):375-81.

Allen KL, Molan PC, Reid GM. A survey of the antibacterial activity of some New Zealand honey. J Pharm Pharmacol 1991;2(43):817-39.

Mavric E, Wittmann S, Barth G, Henle T. Identification and quantification of methylglyoxal as the dominant antibacterial constituents of Manuka (Leptospermum scoparium) honey from New Zealand. J Mol Nut Food Res 2008;9(52):483-92.

Yap P, Lim H, Carrier D, Abu Bakar MF. Antibacterial activity of polyphenol rich extract of selected wild honey collected in Sabah, Malaysia. J Api Res 2014;(in press)

Melissa AM, Olga IPZ, Randy WW. Growth inhibition of food borne pathogens and food spoilage organisms by select raw honey. Int J Food Microbiol 2004;11(97):1-8.

Sabli F, Mohamed M, Rahmat A, Ibrahim H, Abu Bakar MF. Antioxidant properties of selected Etlingera and Zingiber species (Zingiberaceae) from Borneo Island. Int J Bio Chem 2012;6:1-9.

Abu Bakar MF, Teh AH, Rahmat A, Hashim N, Othman F, Fakurazi S. Antiproliferative properties and antioxidant activity of various types of Strobilanthes crispus tea. Int J Can Res 2006;2 (2):152-8.

Published

01-07-2014

How to Cite

Yap, P., and M. F. Abu Bakar. “PHYSICOCHEMICAL, PHYTOCHEMICAL AND ANTIMICROBIAL PROPERTIES OF WILD HONEY COLLECTED AT MANGROVE AND MOUNTAIN AREAS IN SABAH, MALAYSIAN BORNEO”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 287-92, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2008.

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