IN VITRO ANTIDIABETIC, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF CLITORIA TERNATEA L
Keywords:Clitoria ternaea L, Antioxidant, Phytochemicals, Antidiabetic, Anti-inflammatory
Objective: To investigate the in vitro Antidiabetic, Antioxidant and Anti-inflammatory activity of Clitoria ternatea L. leaves and flowers.
Methods: Phytochemicals of leaves and flowers were analysed by using standard methods. In vitro antioxidant studies were carried out for the ethanolic extract of the Clitoria ternatea leaves and flowers using various free radical models such a DPPH, Reducing power assay and Hydrogen peroxide scavenging assay. In vitro antidiabetic assay such as Non-enzymatic glycosylation of haemoglobin assay, Glucose uptake in Yeast cells and Inhibition of salivary-amylase enzyme were carried in ethanolic extract.. In vitro anti-inflammatory activity such as inhibition of albumin denaturation and membrane stabilization assay were performed in ethanolic extract.
Results: Preliminary phytochemical screening of ethanolic extract of the Clitoria ternatea revealed the presence of various bioactive components like alkaloids, flavonoids, steroids, glycosides, phenol, saponin, terpenoids and tannin in both leaves and flowers. Anthroquinone is absent in both the parts studied. The in vitro antidiabetic potential of plant extract was confirmed through non enzymatic glycation, glucose uptake by yeast cells and amylase inhibition methods. Antiinflammatory activity of Clitoria ternaea was also confirmed.
Conclusion: The result of the present study concluded that the ethanolic extracts of Clitoria ternaea L. leaves and flowers possess significant antidiabetic, antioxidant and anti-inflammatory activity. The potential pharmacological activity of Clitoria ternaea L. leaves and flowers might be due to the presence of phytochemicals.
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