EFFICACY OF CLITORIA TERNATEA LEAF EXTRACT AGAINST EXPERIMENTAL ASPERGILLOSIS
Keywords:Aspergillosis, Immunocompromised mice, Clitoria ternatea extract, Galactomannan, Fungal burden
Objective: To evaluate the efficacy of methanolic extract of Clitoria ternatea leaf in experimental murine systemic aspergillosis.
Methods: The investigation of methanolic extract of C. ternatea leaf against experimental murine systemic aspergillosis was assessed by the survival rates, quantitative determination of fungal burden in spleen, kidney and lung organs, histopathological evaluation and serum galactomannan index.
Results: Methanolic extract of C. ternatea and amphotericin B demonstrated survival benefits over control. All untreated animals died by day 14. Similarly, both treated groups had significant reductions (P< 0.05) in the fungal burden of lungs, kidneys and spleen. By day 28, there was a complete clearance of the fungi from these organs. A decline was also observed in serum galactomannan level of treated animals.
Conclusion: The methanolic extract of C. ternatea was effective against aspergillosis in immunocompromised mice comparable to that of amphotericin. This study merits further clinical investigations of this extract as well as isolation and characterization of their bioactive antifungal chemical constituent(s).
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