EFFICACY OF CLITORIA TERNATEA LEAF EXTRACT AGAINST EXPERIMENTAL ASPERGILLOSIS

Authors

  • Linggam Kamilla Center for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Surash Ramanathan Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Sreenivasan Sasidharan Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Vikneswaran Murugaiyah School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • Sharif Mahsufi Mansor Center for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

Keywords:

Aspergillosis, Immunocompromised mice, Clitoria ternatea extract, Galactomannan, Fungal burden

Abstract

Objective: To evaluate the efficacy of methanolic extract of Clitoria ternatea leaf in experimental murine systemic aspergillosis.

Methods: The investigation of methanolic extract of C. ternatea leaf against experimental murine systemic aspergillosis was assessed by the survival rates, quantitative determination of fungal burden in spleen, kidney and lung organs, histopathological evaluation and serum galactomannan index.

Results: Methanolic extract of C. ternatea and amphotericin B demonstrated survival benefits over control. All untreated animals died by day 14. Similarly, both treated groups had significant reductions (P< 0.05) in the fungal burden of lungs, kidneys and spleen. By day 28, there was a complete clearance of the fungi from these organs. A decline was also observed in serum galactomannan level of treated animals.

Conclusion: The methanolic extract of C. ternatea was effective against aspergillosis in immunocompromised mice comparable to that of amphotericin. This study merits further clinical investigations of this extract as well as isolation and characterization of their bioactive antifungal chemical constituent(s).

Downloads

Download data is not yet available.

References

Hatziagorou E, Walsh TJ, Tsanakas JN, Roilides E. Aspergillus and the paediatric lung. Paediatr Resp Rev 2009;10:178-85.

Denning DW. Invasive aspergillosis. Clin Infect Dis 1998;26:781-803.

Kontoyiannis DP, Bodey GP. Invasive aspergillosis in 2002: an update. Eur J Clin Microbiol Infect Dis 2002;21:161-72.

Kontoyiannis DP, Mantadakis E, Samonis G. Systemic mycoses in the immunocompromised host: an update in antifungal therapy. J Hosp Infect 2003;53:243-58.

Young RC, Bennett JE, Vogel CL, Carbone PP, DeVita VT. Aspergillosis: the spectrum of disease in 98 patients. Med 1970;49:147-73.

Bode FR, Pare JAP, Fraser RG. Pulmonary diseases in the compromised host. Med 1974;53:255-93.

Lonneux M, Nolard N, Philippart I, Henkinbrant A, Hamels J, Fastrez J, et al. A case of lymphatic pneumonitis, myositis and arthritis associated with exposure to Aspergillus niger. J Allergy Clin Immunol 1995;95:1047-9.

Niwano Y, Kuzuhara N, Goto Y, Munechika Y, Kodama H, Kanai K, et al. Efficacy of NND 502, a novel imidazole and antimycotic agent, in experimental models of Candida albicans and Aspergillus fumigatus infections. Int J Antimicrob Ag 1999;12:221-8.

Sionov E, Mendlovic S, Segal E. Efficacy of amphotericin B and Amphotericin B intralipid in combination with caspofungin against experimental aspergillosis. Brit Infect Soc 2006;53:131-9.

Schmourlo G, Mendong RR, Filho A, Alviano CC. Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants. J Ethnopharmacol 2005;96:563-8.

Mukherjee PK, Kumar V, Kumar NS, Heinrich M. The Ayurvedic medicine Clitoria ternatea from traditional use to scientific assessment. J Ethnopharmacol 2008;120:291-301.

Kamila L, Mansor SM, Ramanathan S, Sasidharan S. Effects of Clitoria ternatea leaf extract on growth and morphogenesis of Aspergillus niger. Microsc Microanal 2009;15:1-7.

Kamila L, Sasidharan S, Ramanathan S, Mansor SM. Toxicity evaluation of methanol extract of Clitoria ternatea L. leaf. Malays J Med Health Sci 2012;8:33-9.

Sionov E, Segal E. Polyene and cytokine treatment of experimental aspergillosis. FEMS Immunol Med Microbiol 2003;39:221-7.

Latge JP. Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev 1999;12:310-50.

White PL, Archer AE, Barnes RA. Comparison of non-culture based methods for detection of systemic fungal infections with an emphasis on invasive candida infections. J Clin Microbiol 2005;43:2181-7.

Becker MJ, de Marie S, Willemse D, Verbrugh HA, Barker-Woudenberg IAJM. Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model. J Clin Microbiol 2000;38:1434-8.

Published

01-11-2014

How to Cite

Kamilla, L., S. Ramanathan, S. Sasidharan, V. Murugaiyah, and S. M. Mansor. “EFFICACY OF CLITORIA TERNATEA LEAF EXTRACT AGAINST EXPERIMENTAL ASPERGILLOSIS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 11, Nov. 2014, pp. 514-6, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2014.

Issue

Section

Short Communication(s)

Most read articles by the same author(s)