EFFECTS OF ORALLY ADMINISTERED ENTEROCOCCUS FAECIUM ON THE IMMUNE SYSTEMIC RESPONSE AND INTESTINAL EPITHELIAL STRUCTURE IN BALB/C MICE IMMUNIZED BY BOVINE Î’-LACTOGLOBULIN

Authors

  • Wafaa Dib Laboratory of Nutrition Physiology and Food Safety, Department of Biology, Faculty of Science, University of Oran, El Menaouer 31000 Oran, Algeria
  • Abdallah Chekroun Laboratory of Nutrition Physiology and Food Safety, Department of Biology, Faculty of Science, University of Oran, El Menaouer 31000 Oran, Algeria
  • Shady El-Ghaish Department of Dairy Sciences, Faculty of Agriculture, Kafrelsheikh University, B.P. 33516, Egypt
  • Yvan Choiset UR 1268 Biopolymere, Interactions, Assemblage, equipe FIP, INRA, 44300 Nantes, France.
  • Jean-Marc Chobert UR 1268 Biopolymere, Interactions, Assemblage, equipe FIP, INRA, 44300 Nantes, France.
  • Thomas Haertle UR 1268 Biopolymere, Interactions, Assemblage, equipe FIP, INRA, 44300 Nantes, France.
  • Djamel Saidi Laboratory of Nutrition Physiology and Food Safety, Department of Biology, Faculty of Science, University of Oran, El Menaouer 31000 Oran, Algeria
  • Omar Kheroua Laboratory of Nutrition Physiology and Food Safety, Department of Biology, Faculty of Science, University of Oran, El Menaouer 31000 Oran, Algeria

Keywords:

Enterococcus faecium, probiotic, Nil, Immunoglobulin G, Intestinal epithelium

Abstract

Objective: Determine the effect of Enterococcus faecium on systemic immune response and its effect on the intestinal epithelial structure in the Balb/c mice.

Methods: Thirty Balb/c mice were dispatched in three lots of 10 mice each. During an initial period of 18 days, the animals from the first lot received via an oral way suspension of 0,3 mL containing 108 ufc/mL of Enterococcus faecium,for the second and the third lot received 0,3 mL of a saline solution. In a second period of time, mice from the first and second lots were immunised via parenteral way using β-Lg. Then they were sacrified on the 50th day after the end of the first period (18 days). The level of IgG anti-β-Lg was determined in the sera by the ELISA, and histologic studies were conducted on the jejunum fragments.

Results: Our results show that anti β-Lactoglobulin IgG titers were significantly reduced in immunized mice that received the Enterococcus faecium (1/280th) (***p<0,001). The histological studies of the intestinal epithelium shows long intestinal villi (53,88 ± 1,38µm) with diminished intra-epithelial lymphocytes.

Conclusion: The study shows that Enterococcus faecium PC4.1 may help protect the intestinal epithelium integrity by maintaining the structure of the villi and has the ability to decrease the systemic immune response to β-lactoglobulin.

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Published

01-07-2014

How to Cite

Dib, W., A. Chekroun, S. El-Ghaish, Y. Choiset, J.-M. Chobert, T. Haertle, D. Saidi, and O. Kheroua. “EFFECTS OF ORALLY ADMINISTERED ENTEROCOCCUS FAECIUM ON THE IMMUNE SYSTEMIC RESPONSE AND INTESTINAL EPITHELIAL STRUCTURE IN BALB/C MICE IMMUNIZED BY BOVINE Î’-LACTOGLOBULIN”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 587-90, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2024.

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