CHEMICAL COMPOSITION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OIL FROM LIME BASIL (OCIMUM AMERICANUM): A POTENT SOURCE FOR NATURAL ANTIOXIDANT
Keywords:Ocimum americanum, GC MS analysis, Antioxidant activity, Antimicrobial activity
Objective: GC and GC MS based analysis of the essential oil from leaf samples of Ocimum americanum were done. The essential oil composition, In vitro antioxidant and antimicrobial activity of the essential oil of O. americanum were evaluated in this research to explore its potential for future use.
Methods: The component identification was achieved by the GCâ€“MS analysis. Radical-scavenging activities of the plant essential oil were determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) activity. Antimicrobial activity of the essential oil was done using standard pathogenic strains in disc diffusion method.
Results: GC and GC/MS analysis of the plant essential oil resulted in the identification of 19 compounds representing 100% of the oil. Citral (47.18%) and Î² - citral (36.57%) were the two major constituents. The essential oil subjected to analysis of its antioxidant activity using 2, 2- diphenyl-1-1picrylhydrazyl (DPPH) showed higher activity as compared to the standard. The oil was also screened for its antimicrobial activity and the inhibition was recorded against pathogenic microorganisms.
Conclusion: Both the antimicrobial and anti oxidant activity of Ocimum americanum showed its potential for further use as natural antioxidant and preservative. Essential oil from the medicinal plants is one of the promising natural compounds for preparation of safer antimicrobial and antioxidant agent. Antioxidants conventionally used in food industries are obtained by chemical synthesis, but their use, reserved for certain categories of food is regulated by dose limits. Leaves of Ocimum americanum L. contain citral rich essential oil which is a potent source for natural antioxidants and biologically active compounds.
Kazembe TC, Chaibva M. Mosquito repellency of whole extracts and volatile oils of Ocimum americanum, Jatropha curcas and Citrus limon. Bulletin of Environment Pharmacology and Life sciences p. 2012;1(8):64-71.
Aluko BT, Oloyede OI, Afolayan AJ. Polyphenolic contents and free radical scavenging potential of extracts from leaves of Ocimum americanum L. Pakistan J of Biological Sci PJBS 2013;16(1):22-30.
Bassole IHN, Ouattara CT, Traore SA, Nebie R, Savadogo A, Barro N, et al. Composition and antimicrobial activities of the leaf and flower essential oils of Lippia chevalieri and Ocimum canum from Bukina faso. Afr p. 2005;4(10):1156-60.
Wang J, Zhao J, Liu H, Zhou L, Liu Z, Wang J, et al. Chemical analysis and biological activity of the essential oils of two valerianaceous species from China:Nardostachys chinensis and Valeriana officinalis. Molecules (Basel, Switzerland). 2010;15(9):6411-22.
Hussain AI, Sherazi STH, Anwar F, Roman P. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depend on seasonal variations. J Food Chemistry 2008;108:986-95.
Bozin B, Mimica-Dukic N, Simin N, Anackov G. Characterization of the volatile composition of essential oils of some lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. J Agric Food Chem 2006;54(5):1822-8.
Celiktas OY, Kocabas EEH, Sukan FV, Baser KHC, Bedir E, Ozek T. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus oficinalis, depending on location and seasonal variations. J Food Chemistry 2007;100(2):553-9.
Guenther E, Robert E, New K. The production of essential oils1972. 361-91p.
Washington JA. Laboratory procedures in clinical microbiology. J New York Springer Verlag 1981.
Haung D, Ou B, Prior RL, J. The chemistry behind antioxidant capacity assays. J Chem 2005;53:1841-56.
Sarker SD, Gray AI, Latif Z. Natural Products Isolation, second ed 2006.
Singh S, Tewari G, Pande C, Singh C. Variation in essential oil composition of Ocimum americanum L. from northwestern Himalayan region. J of Essential Oil Res 2013;25(4):278-90.
Plummer JA, Wann JM, Spadek ZE. Intraspecific variation in oil components of Boronia megastima Nees. Rutaceae Flowers Annals of Botany p 1999;83:253-62.
Kpadonou Kpoviessi BGH, Ladekan EY, Kpoviessi DSS, Gbaguidi F, Yehouenou B, Quetin-Leclercq J, et al. Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach. J Chem Biodivers 2012;9(1):139-50.
Yavari A, Shahgolzari SM, J. Essential oil variation in the population of Stachys inflate Benth. J From Iran American Eurasian and Environ Science 2013;13(4):461-9.
Thaweboon S., Boonyanit B. 2009. In vitro antimicrobial activity of Ocimum americanum L. essential oil against oral microorganisms. The Southeast. Asian J of Tropical Medicine and Public Health 40(5):1025-33.
Mao LC, Fang XH, Pan X, Que F. Antioxidant properties of water and ethanol extracts from hot air-dried and freeze dried daylily flowers. J Eur Food Res Technol 2006;222:236-41.
Soares JR, Dinis TC, Cunha AP, Almeida LM. Antioxidant activities of some extracts of Thymus zygis. J Free Radic Res 1997;26(5):469-78.
Hsu C-Y, Chan Y-P, Chang J. Antioxidant activity of extract from Polygonum cuspidatum. J Biol Res 2007;40(1):13-21.
Jayasinghe C, Gotoh N, Aoki T, Wada S. Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). J Agric Food Chem 2003;51(15):4442-9.
George S, Chaturvedi P. A comparative study of the antioxidant properties of two different species of Ocimum of southern Africa on alcohol-induced oxidative stress. J Med Food 2009;12(5):1154-8.
Ramesh B, Satakopan VN. Antioxidant Activities of Hydroalcoholic Extract of Ocimum sanctum Against Cadmium Induced Toxicity in Rats. Indian J of Clinical Biochemistry IJCB 2010;25(3):307-10.
Thaweboon S, Thaweboon B. In vitro antimicrobial activity of Ocimum americanum L. essential oil against oral microorganisms. The Southeast Asian J of Tropical Medicine and Public Health 2009;40(5):1025-33.
Singh S, Taneja M, Majumdar DK. Biological activities of Ocimum sanctum L. fixed oil--an overview. Indian J Exp Biol 2007;45(5):403-12.