PROTECTIVE ROLE OF BACOPA MONNIERI ON INDUCED PARKINSON'S DISEASE WITH PARTICULAR REFERENCE TO CATECHOLAMINE SYSTEM
Keywords:Parkinson`s disease (PD), Bacopa monnieri (BM), Rotenone (RT), Levodopa (LD), Dopamine (DA), Serotonin (5-HT), Epineprine (EP), Nor-epineprine (NEP) and Monoamine oxidase (MAO)
Objective: Parkinson's disease (PD) is the second most neurodegenerative disorder. Bacopa monnieri (BM), an Indian herb extensively used in Ayurveda, used in treatments related to neurological complications was used now in our study.
Methods: In the present experiment, rats were divided into four groups of six in each group: group 1 received saline water, group 2 received rotenone (RT) through i.p. for 60 days to induce PD, group 3 received for 20 days orally before induction of PD and group 4 received Levodopa (Reference drug; LD) orally.
Results: The levels Dopamine (DA), Serotonin (5-HT), Epineprine (EP), Nor-epineprine (NEP) were decreased and Monoamine oxidase (MAO) activity was increased in different brain regions such as Cerebral cortex (CC), Cerebellum (CB), Mid brain (MB) and Pons medulla (PM) during induced PD compared with controls. These results were reversed after treatment with ethanolic extract of BM on par with reference drug (LD).
Conclusion: Our results suggest the ability of BM extract to modulate catecholamine system in different brain regions of RT induced rodent model of PD and thus offers protection. When compared overall the BM is better than the LD drug. The BM may provide a platform for future drug discoveries and novel treatment strategies in PD and can act as antiparkinsonian agent.
Aarsland D, Bronnick K, Ehrt U et al. Depressive symptom profile in Parkinsonâ€™s disease:a comparison with depression in elderly patients with Parkinsonâ€™s disease. Int J of Geriatric Psychiatry 2006;21:252-8.
Ahlskog J E et al. Levodopa and deprenyl treatment effects on peripheral indices of oxidant stress in Parkinsonâ€™s disease. J Neurology 1996;46(3):796-801.
Alam M, Schmidt W J. Rotenone destroys dopaminergic neurons and induces parkinsonian symptoms in rats. J Behav Brain Res 2002;136(1):317-24.
Alam M, Schmidt W J. L-DOPA reverses the hypokinetic behaviour and rigidity in rotenone-treated rats. J Behav Brain Res 2004;153 (2), 439-46.
Bastianetto S, Zheng W H, Quirion R. The Ginkgo biloba extract (Egb 761) protects and rescues hippocampal cells against nitric oxide-induced toxicity involvement of its flavonoid constituents and protein kinase C. J Neuro Chem 2002;74:2268-77.
Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV, Greenamyre JT. Chronic systemic pesticide exposure reproduces features of Parkinson's disease. J Nat Neuro sci 2000;3:1301-06. [Pub Med:11100151]
Bhattacharya S K, Bhattacharya A, Kumar A, Ghosal S. Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. J Phytotherapy Res 2000;14(3), 174-9.
Brodie B B, Costa E, Dlabac A, Neff N H, Smookler H H. Application of steady state kinetics to the estimation of synthesis rate and turnover time of tissue catecholamines. J Pharmacol Exp Ther 1966;154:493-8.
Chatterjee N, Rastogi R P, Dhar M L. Chemical examination of Bacopa monniera Wettst:Part I. Isolation of chemical constituents. Indian J Chem 1963;1:212-5.
Chatterjee N, Rastogi R P, Dhar M L. Chemical examination of Bacopa monniera Wettst:Part II. The constitution of bacoside A. Indian J Chem 1965;3:24-9.
Das A, Shanker G, Nath C, Pal R, Singh S, Singh H K. A comparative study in rodents of standardized extracts of Bacopa monniera and Ginkgo biloba anticholinesterase and cognitive enhancing activities. J Pharmacol Biochem Behav 2002;73:893-900.
Deogaonkar M, Subramanian T. Pathophysiological basis of drug-induced dyskinesias in Parkinsonâ€™s disease. J Brain Res Rev 2005;50:156-68.
Green A L, Haughton T M. A colorimetric method for the estimation of monoamine oxidase. J Biochem 1961;78:172-5.
Kari H P, Davidson P P, Herbert H H, Kochhar M H. Effect of ketamine on brain monoamines levels in rats. J Res Comm Chem Path Pharmacol 1978;20:475-88.
Kellstein D E, Malseed R T, Ossipov M H, Goldstein F J. Effect of chronic treatment with tricyclic antidepressants upon antinociception induced by intrathecal injection of morphine and monoamines. J Neuropharmacolog 1988;27:1-14.
Rao H, Gillihan S J, Wang J, Korczy kowski M, Sankoorikal G M, Kaercher K A, Brodkin E S, Detre J A, Farah M J. Genetic Variation in Serotonin Transporter Impacts Default Amygdala Function in Healthy Brain. J Biological Psychiatry 2007;62(6):600-06.
Sairam K, Rao C V, Babu M D, Goel R K. Prophylactic and curative effects of Bacopa monniera in gastric ulcer models. J Phytomedicine 2001;8:423-30.
Schmidt W J, Alam M. Controversies on new animal models of Parkinsonâ€™s disease pro and con:the rotenone model of Parkinsonâ€™s disease (PD). J Neural Transm 2006;70(Suppl):273-6.
Sheikh N, Ahmad A, Siripurapu K B et al. Effect of Bacopa monniera on stress induced changes in plasma corticosterone andbrain monoamines in rats. J Ethnopharmacol 2007;111:671-6.
Sherer T B, Betarbet R, Stout A K, Lund S, Baptista M, Panov A V, Cookson M R, Greenamyre J T. An in vitro model of Parkinsonâ€™s disease:linking mitochondrial impairment to altered-synuclein metabolism and oxidative damage. J Neurosci 2002;22:7006-7015.
Sherer T B, Kim J H, Betarbet R, Greenamyre J T. Subcutaneous rotenone exposure causes highly selective dopaminergic degeneration and alpha-synuclein aggregation. J Exp Neurol 2003;179:9-16.
Shetty P, Atallah, M T, Shetty K. Effects of UV treatment on the proline-linked pentose phosphate pathway for phenolics and L-DOPA synthesis in dark germinated Vicia faba. J Process Biochem 2002;37(11):1285-95.
Sipos I, Tretter L, Adam-Vizi V. Quantitative relationship between inhibition of respiratory complexes and formation of reactive oxygen species in isolated nerve terminals. J Neurochem 2003;84:112-8.
Swathi G, Bhuvaneswar C, and Rajendra, W. Alterations of Cholinergic neurotransmission in Rotenone induced Parkinsonâ€™s disease:protective role of Bacopa monnieri. IJPBS.20;3(2):286-292.
Talpade D J, Greene J G, Higgins D S, Greenamyre J T. In vivo labeling of mitochondrial complex I (NADH:ubiquinone oxidoreductase) in rat brain using [(3)H] dihydrorotenone. J Neurochem 2000;75:2611-21.