CHARACTERIZATION OF 2,4,6-TRINITROTOLUENE (TNT)-METABOLIZING STRAIN PSEUDOMONAS AERUGINOSA SP TUHP1 ISOLATED FROM TNT-POLLUTED SOILS IN THEVELLORE DISTRICT, TAMILNADU,INDIA

Authors

  • Hannah Elizabeth S. Department of Zoology, Thiruvalluvar University, Serkadu, Vellore, Tamil Nadu, India.
  • Panneer Selvam A. Department of Zoology, Thiruvalluvar University, Serkadu, Vellore, Tamil Nadu, India.

Keywords:

Bioremediation, Biodegradation, Biotransformation, Sequencing

Abstract

Objective: The main objective was to evaluate the degradative properties of Pseudomonas aeruginosa sp TUHP1 isolated from TNT-Polluted soils in the Vellore District, Tamil Nadu, India.

Methods: Among the 3 bacterial genera isolated from different soil samples, one potent TNT degrading strain Pseudomonas aeruginosa sp TUHP1 was identified. The morphological, physiological and the biochemical properties of the strain Pseudomonas aeruginosa sp TUHP1 was confirmed by conventional methods and genotypic characterization was carried out using 16S r-DNA partial gene amplification and sequencing. The broken down by products of DNT in the extract was determined by Gas Chromatogram- Mass spectrometry (GC-MS). Supernatant samples from the broth studied at 24 h interval were analyzed by HPLC analysis and the effect on various nutritional and environmental factors were analysed and optimized for the isolate.

Results: Out of three isolates one strain TUHP1 were found to have potent efficiency to degrade TNT and revealed the genus Pseudomonas. 16S rDNA gene sequence analysis showed highest homology (99%) with Pseudomonas aeruginosa DS10 and was assigned as Pseudomonas aeruginosa sp TUHP1. Based on the energy of the predicted models, the secondary structure predicted by MFE showed the more stable structure with a minimum energy. Products of TNT Transformation showed colour change in the medium during cultivation. TNT derivates such as 2HADNT and 4HADNT were detected by HPLC chromatogram and 2ADNT, 4ADNT by GC/MS analysis.

Conclusion: Hence this study presents the clear evidence for the biodegradation process of TNT by strain Pseudomonas aeruginosa sp TUHP1.

Keywords: Bioremediation. Biodegradation. Biotransformation. Sequencing.

 

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References

Nathan A, Jessica M, Bernan V, Dworkin M, H. D. Magarvey, Keller, Sherman and characterization of novel marine derived actinomycete taxa rich in bioactive metabolites. J Appl Environ Microbiol 2004;70:7520-9.

Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E. The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage:proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 1996;46(4):1088-92.

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs. J Nucleic Acids Res 1997;25(17):3389-402.

Benson DA, Boguski MS, Lipman DJ, Ostell J, Ouellette BF, Rapp BA, et al. Gen Bank. J Nucleic Acids Res 1999;27(1):12-7.

Shenghan Z. Huangkai Wei-Hua Chen. EvolView an online tool for visualizing annotating and managing phylogenetic trees. J Nucleic acids Res 2012;40:569-72.

Boopathy R, Wilson M, Montemagno CD, Jnr JF, Kulpa CF. Manning Biological transformation of 2,4,6-trinitrotoluene (TNT) by soil bacteria isolated from TNT-contaminated soil. J Bioresource Technology 1994;47:19-24.

Shen J, Zhang J, Zuo Y, Wang L, Sun X, Li J, et al. Biodegradation of 2,4,6-trinitrophenol by Rhodococcus sp. isolated from a picric acid-contaminated soil. J Hazard Mater 2009;163(2-3):1199-206.

Snell FD. C.T. Snell. Colorimetic methods of analysis,;vol. pp.. Van Nostrand Co. J Inc New York. 1949;3:804-5.

Dillewijn PV, Caballero A. Bioremediation of 2,4,6-trinitrotoluene under field conditions. J Environmental Sci Technology 2007;41:1378-83.

Drzyzga O, Bruns-Nagel D, Gorontzy T, Blotevogel KH, Gemsa D, von Löw E. Mass balance studies with 14C-labeled 2,4,6-trinitrotoluene (TNT) mediated by an Anaerobic desulfovibrio species and an Aerobic serratia species. J Curr Microbiol 1998;37(6):380-6.

Naine S, Nasimunislam N, Vaishnavi B, Mohanasrinivasan V, Devi C. Jemimah Subathra Isolation of soil actinomycetes inhabiting amirithi forest for the potential source of bioactive compounds. Asian J Pharm Clin Res 2012;5(4):189-92.

Spain JC, Hughes JB, Lewis E, C. CR. KnackmussHJ. (Eds.). J Biodegradation of Nitroaromatics and Boca Raton 2000.

Esteve-Nunez A, Caballero A, Ramos JL. Biological degradation of 2,4,6-trintitrotoluene, Microbiology and Molecular Biology Reviews. J Appl Environ Microbiol 2001;65:335-52.

Snellinx Z, Nepovím A, Taghavi S, Vangronsveld J, Vanek T, van der Lelie D. Biological remediation of explosives and related nitroaromatic compounds. J Environ Sci Pollut Res Int 2002;9(1):48-61.

Nies DH. Microbial heavy-metal resistance. Appl Microbiol Biotechnol. 1999;51(6):730-50.

Hong, YC, Azad, HR, Cooksey, DA. A chromosomal locus required for copper resistance, competitivefitness and cytochrome c biogenesis in Pseudomonas fluorescens. Proceedings of the National Academy of Sciences of the United States of America. 1996;93:7315-7320.

Koropatnick J, Zalups RK. Effect of non-toxic mercury, zinc or cadmium pretreatment on the capacity of human monocytes to undergo lipopolysaccharide-induced activation. Br J Pharmacol 1997;120(5):797-806.

Maeda T, Nakamura R, Kadokami K, Ogawa HI. Relationship between mutagenicity and reactivity or biodegradability for nitroaromatic compounds. Environmental. J Toxicology and Chemistry. 2007;26:237e241.

Gomaa EZ. Chitinase production by Bacillus thuringiensis and Bacillus licheniformis:their potential in antifungal biocontrol. J of Microbiology (Seoul, Korea) 2012;50(1):103-11.

Gruber AR, Lorenz R, Bernhart SH, Neuböck R, Hofacker IL. J Vienna RNA Websuite Nucleic Acids Res, 2008;36.

Ding Y, Lawrence CE. A statistical sampling algorithm for RNA secondary structure prediction. J Nucleic Acids Res 2003;31(24):7280-301.

Zuker M, Stiegler P. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. J Nucleic Acids Res 1981;9(1):133-48

Published

01-07-2014

How to Cite

Elizabeth S., H., and P. Selvam A. “CHARACTERIZATION OF 2,4,6-TRINITROTOLUENE (TNT)-METABOLIZING STRAIN PSEUDOMONAS AERUGINOSA SP TUHP1 ISOLATED FROM TNT-POLLUTED SOILS IN THEVELLORE DISTRICT, TAMILNADU,INDIA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 267-71, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2047.

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