IN VITRO HEPATOPROTECTIVE ACTIVITY OF EXTRACTS OF VIBURNUM PUNCTATUM BUCH-HAM EX D.DON AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY
Keywords:Viburnum punctatum, MTT, Silymarin, Hepatoprotective
Objective: The study was aimed to evaluate of in vitro hepatoprotective activity of Chloroform and Methanol extracts of Viburnum punctatum (200 and 400 Î¼g/ml) against carbon tetrachloride induced toxicity.
Methods: The screening of hepatoprotective activity was based on the protection of human liver derived Chang liver cells against CCl4 induced damage determined by MTT assay [(3-(4,5 dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay] using Silymarin as standard.
Results: The chang liver cells were treated with different concentrations of chloroform and methanol extracts of Viburnum punctatum, showed a dose dependent increase in percentage viability and the results were highly significant (P<0.001, when compared with CCl4 induced group). The percentage viability ranged between 62 to 84% at 200-400Âµg/ml concentrations. The methanolic extract exhibited more hepatoprotective activity when compared to chloroform extract.
Conclusion: The results clearly demonstrate that Viburnum punctatum possess promising hepatoprotective effects and hence suggests to isolate and identify the active principle involved in the hepatoprotective activity.
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