IN VITRO HEPATOPROTECTIVE ACTIVITY OF EXTRACTS OF VIBURNUM PUNCTATUM BUCH-HAM EX D.DON AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY

Authors

  • A. Renjith Alex Department of Pharmaceutical Chemistry, Aditya Bangalore Institute of Pharmacy Education and Research, # 12, Kogilu Main Road, Yelahanka, Bangalore 560064, Karnataka, India
  • K. Ilango Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM University, Kattankulathur, Chennai 603203, Tamil Nadu, India.
  • Vishwanath A. Boguda Department of Pharmaceutical Chemistry, Aditya Bangalore Institute of Pharmacy Education and Research, # 12, Kogilu Main Road, Yelahanka, Bangalore 560064, Karnataka, India
  • S. Ganesanan Department of Pharmaceutical Chemistry, Aditya Bangalore Institute of Pharmacy Education and Research, # 12, Kogilu Main Road, Yelahanka, Bangalore 560064, Karnataka, India

Keywords:

Viburnum punctatum, MTT, Silymarin, Hepatoprotective

Abstract

Objective: The study was aimed to evaluate of in vitro hepatoprotective activity of Chloroform and Methanol extracts of Viburnum punctatum (200 and 400 μg/ml) against carbon tetrachloride induced toxicity.

Methods: The screening of hepatoprotective activity was based on the protection of human liver derived Chang liver cells against CCl4 induced damage determined by MTT assay [(3-(4,5 dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay] using Silymarin as standard.

Results: The chang liver cells were treated with different concentrations of chloroform and methanol extracts of Viburnum punctatum, showed a dose dependent increase in percentage viability and the results were highly significant (P<0.001, when compared with CCl4 induced group). The percentage viability ranged between 62 to 84% at 200-400µg/ml concentrations. The methanolic extract exhibited more hepatoprotective activity when compared to chloroform extract.

Conclusion: The results clearly demonstrate that Viburnum punctatum possess promising hepatoprotective effects and hence suggests to isolate and identify the active principle involved in the hepatoprotective activity.

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Published

01-07-2014

How to Cite

Alex, A. R., K. Ilango, V. A. Boguda, and S. Ganesanan. “IN VITRO HEPATOPROTECTIVE ACTIVITY OF EXTRACTS OF VIBURNUM PUNCTATUM BUCH-HAM EX D.DON AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 392-4, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2049.

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