ESTABLISHMENT OF CALLUS AND SUSPENSION CULTURE IN SESAMUM INDICUM L
Keywords:
Sesame, Callus induction, Cell suspension culture, Hypocotyl, Plant growth regulatorsAbstract
Objective: Sesame (Sesamum indicum L.) is one of the most important oilseed crops in the world. We intended to evaluate the effects of different explants, carbon sources and growth regulators on callus induction and cell suspension culture of two Iranian sesame cultivars (Yekta and Karaj1).
Methods: The explants (hypocotyl and cotyledon) were cultured on Murashige and Skoog (MS) basal medium supplemented with 30 g/l sucrose, 8 g/l agar, different concentrations and combinations of NAA, 2, 4-D, BAP and Kin hormones. To produce cell suspension culture, callus was excised and transferred to liquid MS medium supplemented with different concentrations of carbon source (glucose and sucrose) and growth regulators of auxins and cytokinins. The cultures were maintained under both dark and light conditions at different rotation speeds of the gyratory shaker.
Results: Callus produced from hypocotyl explants grew faster during 7 to 30 days of culture and after that stabilized at a lower growth rate. Maximum growth rate of suspension culture was achieved from friable green callus in MS liquid medium supplemented with 3 mg/l NAA and 0.6 mg/l BAP using hypocotyl callus.
Conclusion: The optimized cell suspension culture can be used to elicit the production of secondary metabolites, scale-up mass production in bioreactors and gene transformation.
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