PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF ESBL PRODUCING GRAM NEGATIVE BACILLI IN 200 CASES OF URINARY TRACT INFECTIONS
Keywords:Urinary tract infection, Nil, Resistance
Objective: Isolation and identification of Extended Spectrum Beta Lactamases from urinary isolates and to study their drug sensitivity pattern.
Methods: The study was done on 200 patients with suspected urinary tract infection from Rajindra Hospital, Patiala. Urine samples were processed for significant bacteriuria. The antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method as per the CLSI recommendations. ESBL production was tested by using the double-disk approximation test and the combination disk method.
Results: Out of the total of 200 urine samples, there were 84 (42%) culture positive cases, out of which 78 (92.9%) were found to be gram negative bacilli. E. coli was found to be the commonest uropathogen (60.3%). The percentage of ESBL producers was 69.2%. Maximum number of ESBLs were found among E. coli isolates ie. 80.9%. E. coli was found to be sensitive to imipenem (97.9%) followed by nitrofurantoin (91.5%), amikacin (76.6%), piperacillin-tazobactam (68%) and gentamicin (53.2%). It was relatively resistant to ampicillin. Ciprofloxacin showed sensitivity of 29.8% in E. coli.
Conclusion: E. coli and other isolates were more sensitive to impaired. Nitrofurantoin and piperacillin-tazobactam compared to the other antibiotics tested and therefore these may be the drugs of choice for treatment of infections that are caused by ESBLs.
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