ANTIOXIDANT AND HEPATO PROTECTIVE ACTIVITY OF VARIOUS EXTRACTS OF INDIGOFERA BARBERI GAMBLE AGAINST D-GALACTOSAMINE INDUCED TOXICITY IN RATS
Keywords:D-Galactosamine, Silymarin, Indigofera barberi, Biochemical parameters, Ethanolic extract
Objective: To investigate Antioxidant and Hepatoprotective activity of various extracts of Indigofera barberi Gamble against D-Galactosamine induced toxicity in rats.
Methods: Indigofera barberi Gamble is used traditionally as folk system of medicine in India for the treatment of liver diseases, and as a liver tonic. Petroleum ether (PEEIB), chloroform(CEIB) and ethanolic extracts (EEIB) of Indigofera barberi Gamble (aerial part of the plants extracts, 200mg/kg, p. o) was tested for its hepatoprotective effect against D-galactosamine induced hepatic damage in rats at a dose of (400mg/kg, i. p) to induce hepatotoxicity and Silymarin (25 mg/kg, i. p.) was administered as standard drug.
Results: The biochemical parameters of liver tissue LPO, GSH, GPX, GST, SOD and CAT values were significantly (p<0.01) altered against ethanolic extracts treatment of Indigofera barberi Gamble and were comparable with standard drug silymarin.
Conclusion: Animal pretreatment with petroleum ether and chloroform extracts of Indigofera barberi Gamble did not change above mentioned parameters significantly. The histopathological studies also substantiates the results and hence, it can be concluded from the present study that, the ethanolic extracts of Indigofera barberi Gamble has appreciable hepatoprotective potential in albino rats against D-galactosamine induced hepatic damage.
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