ENHANCEMENT AND VALIDATION OF WOUND HEALING ACTIVITY WITH HERBAL GEL FORMULATED FROM SUB-FRACTION OF BUCHNANIA LANZAN SPRENG. BARK EXTRACT

Authors

  • Bina Kumari Mehta Department of Pharmaceutical Science and Technology, Birla Institute of Technology 2275290, Mesra, Ranchi, Jharkhand, India.
  • Ashok Pattnaik Department of Pharmaceutical Science and Technology, Birla Institute of Technology 2275290, Mesra, Ranchi, Jharkhand, India.
  • Ashok Kumar Department of Pharmaceutical Science and Technology, Birla Institute of Technology 2275290, Mesra, Ranchi, Jharkhand, India.

Keywords:

Gel, Wound healing, Spreadability, Incision and excision wound model

Abstract

Objective: The scheme of the present study is to formulate and evaluate herbal gel prepared from sub- fraction of Buchnania lanazan bark extract to augment its wound healing activity.

Methods: Ethyl acetate fraction of Buchanania lanzan bark (Et- BLB) was sub-fractionated by column chromatography using (7:3 methanol: chloroform) solvent system, phytochemical test for Et- BLB and F1- 4 (ethyl acetate sub-fractions 1, 2, 3 & 4 of methanolic extract of BLB); in particular total flavonoid and phenolic content to demonstrate existence of anti-oxidant activity. In-vivo wound healing supporting study mediated by carrageenan induced paw edema as anti inflammatory activity. Herbal gel was formulated incorporating one of the active sub-fractions essentially F4 in two concentrations (1% & 5%). Carbopol 934, propylene glycol 400, methyl paraben & quantity sufficient distilled water was used to prepare herbal gel; then tri-ethanolamine was added to get neutral pH (6.8 - 7) & gel was evaluated for its spreadability, pH, colour, consistency & appearance. Excision & incision models were used to validate and to review enhancement competence of wound healing activity; employing percent wound contraction & tensile strength of wounded skin as parameters. Reference standard used was framycetin sulphate cream.

Results: Total phenolic and flavonoid content of F4 was showing highest flavonoid and phenolic content 83.3 mg/g and 77.8 mg/g respectively. Prepared gel was reddish brown in colour with satisfying spreadability, acceptable appearance along with homogeneity and negligible irritation. Excision and incision animal wound model, groups treated with F4 5 % gel and reference standard exhibited significant increase in percentage of wound contraction as well as up growth in tensile strength with 177 g (P<0.05) and 181.2 g (P<0.01) respectively.

Conclusion: Data from both the models revealed that 5% gel of F4 showed significant & enhanced wound healing.

Downloads

Download data is not yet available.

References

Biswas TK, Mukherjee B. Plant medicines of Indian origin for wound healing activity:a review. The Int J of Lower Extremity Wounds 2003;2(1):25-39.

Mehrotra S, Mitra R. BMEBR. J Biomedical and Environmental Sci 1980;3:338-52.

Jain R, Jain SK. Effect of Buchanania lanzan Spreng. bark extract on cyclophosphamide induced genotoxicity and oxidative stress in mice. Asian Pac J Trop Med 2012;5(3):187-91.

Puri A, Sahai R, Singh KL, Saxena RP, Tandon JS, Saxena KC. Immunostimulant activity of dry fruits and plant materials used in indian traditional medical system for mothers after child birth and invalids. J Ethnopharmacol 2000;71(1-2):89-92.

Warokar AS, Ghante MH, Duragkar NJ, Bhusari KP, J. Anti-inflammmatory and antioxidant activities of methanolic extract of Buchnania lanzan kernel. Indian Educ Res 2010;44(4):363-8.

Kumari A, Kakkar P. Screening of antioxidant potential of selected barks of Indian medicinal plants by multiple in vitro assays. Biomedical and Environmental Sci 2008;21(1):24-9.

Arya R, Babu V, Ilyas M, Nasim KT. Myricetin 3’ – rhamnoside-3-galactoside from buchnania lanzan (Anacardiaceae). Phytochemistry. J Biomedical and Environmental Sci 1992;31(7):2569-70.

Phillips GD, Whitehead RA, Knighton DR. Initiation and pattern of angiogenesis in wound healing in the rat. American J of Anatomy 1991;192(3):257-62.

Das S, Haldar PK, Pramanik G. Formulation and Evaluation of herbal gel containing Clerodendron Infortunatum leaves extract. Int J of Pharm Tech Res 2011;1(3):140-3.

Kulkarni SK, Jain NK. Pharmacological and Pharmacokinetic studies on marketed gel formulation of nimesulide. J Indian Drugs 2001;38(2):63-6.

Saha K, Mukherjee PK, Das J, Pal M, Saha BP. Wound healing activity of Leucas lavandulaefolia Rees. J Ethnopharmacol 1997;56(2):139-44.

Enam A, Afifi F, Hussaini MA. Khalil, Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in pluronic F127 using mice (Mus musculus). J of Ethnopharmacology 2007;109:104-12.

Nema A, Gupta N, Jain UK. Evaluation of wound healing activity of Tinospora Cordifolia Willd. J Der Pharmacia Sincia 2012;1:126-30.

Sadaf F, Saleem R, Ahmed M, Ahmad SI, Navaid ul Z. Healing potential of cream containing extract of Sphaeranthus indicus on dermal wounds in Guinea pigs. J Ethnopharmacol 2006;107(2):161-3.

Lee KH, Tong TG. Study on the mechanism of action of salycylates, retardation of wound healing by aspirin. J of Pharm Sci 1042 1968;57.

Manus M, Mowry JFF, RW. Staining methods, Histological and chemical. Harper & Row/Evanston, New York:London;1965.p.

Gouthamchandra K, Mahmood R, Manjunatha H. Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. J Environ Toxicol Pharmacol 2010;30(1):11-8.

Svensjo T, Pomahac B, Slama J. Yaof, Accelerated healing of full thickness skin wounds in a wet environment. J Plast Reeconstr Surg 602 2000;106.

Senthil RK, Rajkapoor B, Perumal P. Antioxidant activities of Indigofera cassioides Rottl. Ex DC Using various in vitro assay models. Asian Pacific J of Tropical Biomedicine 2012:256-61.

Turner RA, Academic P. Analgesics:Screening Methods in New York;1965.p.

Pareta SK, Pattnaik AK, Kodati D, Patra KC, Harwansh RK. Analgesic and anti-inflammatory activities of Buchnania Lanzan Spreng. Roots Res J of Pharm Biological and chemical Sci 2011;2:419-25.

Mehta SK, Mukherjee S, Jaiprakash B. Anti-inflammatory activity of the methanolic extract of Buchanania lanzan leaves by carrageenan induced rat paw edema method. Int J of Pharm Sci Review and Res 2011;2:144-45.

Published

01-07-2014

How to Cite

Mehta, B. K., A. Pattnaik, and A. Kumar. “ENHANCEMENT AND VALIDATION OF WOUND HEALING ACTIVITY WITH HERBAL GEL FORMULATED FROM SUB-FRACTION OF BUCHNANIA LANZAN SPRENG. BARK EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 281-6, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2231.

Issue

Section

Original Article(s)

Most read articles by the same author(s)