• Raymond R. Tjandrawinata Dexa Laboratories of Biomolecular Sciences (DLBS)
  • Ipang Djunarko Faculty of Pharmacy, Sanata Dharma University, Jogjakarta, Indonesia
  • Fenty Faculty of Pharmacy, Sanata Dharma University, Jogjakarta, Indonesia
  • Phebe Hendra Faculty of Pharmacy, Sanata Dharma University, Jogjakarta, Indonesia


DLBS0533, Anti-inflammation, Mice, Edema, Carragenan


Objective:  DLBS0533 extract is a bioactive fraction obtained from combination of Phaleria macrocarpa and Nigella sativa. This present study aims to observe its potentially anti-inflammatory activities using carragenaan-induced paw edema in mice as animal model.

Methods:  Mice were divided into control, positive control and dose groups. Diclofenac potassium was used as a positive control. Treatment DLBS0533 was given at dose 39, 78 and 156 mg/kg body weight (b. w.). Edema thickness was examined for 6 hours.

Results:  Reduction in edema is shown in 27.26%; 30.71% and 32.72% at dose of 39, 78 and 156 mg/kg b. w., respectively. Comparation between dose groups and positive control group show that dose 156 mg/kg b. w. did not give significantly different. Therefore, dose 39 and 78 mg/kg b. w. also gave anti-inflammatory activities proved by reduction in edema.

Conclusions:  Taken together, DLBS0533 potentially have anti-inflammatory activities.



Download data is not yet available.


Kaushik D, Kumar A, Kaushik P, Rana AC. Analgesic and anti-inflammatory activity of Pinus roxburghii Sarg. Adv Pharmacol Sci 2012;1-6.

Sinatra RS, Jahr JS, Watkins-Pitchford JM, editors. The essence of analgesia and analgesics. London: Cambridge University Press; 2011. p. 229-32.

Hendra R, Ahmad S, Oskoueian E, Sukari A, Shukor MY. Antioxidant, anti-inflammatory and cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff fruit. BMC Complement Altern Med 2011;11:1-10.

Altaf R, Asmawi MZ, Dewa A, Sadikun A, Umar MI. Phytochemistry and medicinal properties of Phaleria macrocarpa (Scheff.) Boerl. extracts. Pharmacogn Rev 2013;7:73-80.

Chehl N, Chipitsyna G, Gong Q, Yeo CJ, Arafat HA. Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic cancer cells. HPB (Oxford) 2009;11(5):373-81.

Seif AA. Nigella sativa reverses osteoporosis in ovariectomized rats. BMC Complement Altern Med 2014;14:22.

Tjandrawinata RR, Arifin PF, Tandrasasmita OM, Rahmi D, Aripin A. DLBS1425:Phaleria macrocarpa (Scheff.) Boerl. extract confers anti-proliferative and proapoptosis effects via eicosanoid pathway. J Exp Ther Oncol 2010;8:187-201.

Tandrasasmita OM, Lee JS, Baek SH, Tjandrawinata RR. Induction of cellular apoptosis in human breast cancer by DLBS1425, a Phaleria macrocarpa compound extract, via downregulation of PI3-kinase/AKT pathway. Cancer Biol Ther 2010;10(8):1-11.

Morris CJ. Carragenan-induced paw edema in the rat and mouse. Methods Mol Biol 2003;225:115-21.

Vogel HG. Drug discovery and evaluation: pharmacological assay. 2nd Ed. Frankfurt; 2002. p. 760-1.

Ma Y, Li Y, Li X, Wu Y. Anti-inflammatory effects of 4-methylcyclopentadecanone on edema models in mice. Int J Mol Sci 2013;14:23980-92.

Posadas I, Bucci M, Roviezzo F, Rossi A, Parente L, Sautebin L, et al. Carrageenan-induced mouse paw oedema is biphasic, age-weight dependent and displays differential nitric oxide cyclooxygenase-2 expression. Br J Pharmacol 2004;142:331-8.

Alemi M, Sabouni F, Sanjarian F, Haghbeen K, Ansari S. Anti-inflammatory effect of seeds and callus of Nigella sativa L. extracts on mix glial cells with regard to their thymoquinone content. APPS Pharm Sci Tech 2013;14(1):160-7.

Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 2005;5(13-14):1749-70.

Mansour M, Tornhamre S. Inhibition of 5-lipoxygenase and leukotriene C4 synthase in human blood cells by thymoquinone. J Enzyme Inhib Med Chem 2004;19(5):431-6.

Ghannadi A, Hajhashemi V, Jafarabadi H. An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J Med Food 2005;8(4):488-93.

Korhonen R, Lahti A, Kankaanranta H, Moilanen E. Nitric oxide production and signaling in inflammation. Curr Drug Targets Inflam Allergy 2005;4:471-9.

Cirino G, Distrutti E, Wallace JL. Nitric oxide and inflammation. Inflam Allergy Drug Targets 2006;5(2):115-9.

Lay MM, Karsani SA, Mohajer S, Malek SNA. Phytochemical constituents, nutritional values, phenolics, flavonols, flavonoids, antioxidant and cytotoxicity studies on Phaleria macrocarpa (Scheff.) Boerl fruits. BMC Complement Altern Med 2014;14:152.

Harris GK, Qian Y, Leonard SS, Sbarra DC, Shi X. Luteolin and chrysin differentially inhibit cyclooxygenase-2 expression and scavenge reactive oxygen species but similarly inhibit PGE-E2 formation in RAW 264.7 cells. J Nutr 2006;136(6);1517-21.



How to Cite

Tjandrawinata, R. R., I. Djunarko, Fenty, and P. Hendra. “ANTI-INFLAMMATION EFFECTS OF BIOACTIVE FRACTION DLBS0533 CONTAINING PHALERIA MACROCARPA AND NIGELLA SATIVA ON ANIMAL MODEL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 1, Jan. 2015, pp. 408-11,



Original Article(s)

Most read articles by the same author(s)