• Abd Almonem Doolaanea Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Malaysia
  • Ahmad Fahmi Harun Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Malaysia
  • Farahidah Mohamed Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Malaysia. International Institute of Halal Research & Training (INHART), Ground Floor, Block E0, Kulliyyah of Engineering, IIUM, P. O. Box 10, 50728, Kuala Lumpur, Malaysia. IKOP Sdn. Bhd., Pilot Plant Pharmaceutical Manufacturing, Faculty of Pharmacy, IIUM, 25200 Kuantan, Malaysia.


Nigella sativa, FTIR, Loading efficiency, PLGA, Chitosan, Nanoparticle


Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent.

Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations.

Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage.


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How to Cite

Doolaanea, A. A., A. F. Harun, and F. Mohamed. “QUANTIFICATION OF NIGELLA SATIVA OIL (NSO) FROM BIODEGRADABLE PLGA NANOPARTICLES USING FTIR SPECTROSCOPY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 10, Oct. 2014, pp. 228-32, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/2529.



Original Article(s)