ANTIMICROBIAL ACTIVITY SCREENING OF M. MINUTA EXTRACTS
Keywords:M minuta, Antimicrobial activity, Infectious disease, Traditional drugs
Objective: Infectious diseases are a leading cause of global disease burden with high morbidity and mortality especially in the developing world. Furthermore, there have been threats of new diseases during the past three decades due to the evolution and adaptation of microbes and the re-emergence of old diseases due to the development of antimicrobial resistance and the capacity to spread to new geographic areas. Since antiquity, humans have used plants to treat common infectious diseases and some of these traditional medicines are still included as part of the habitual treatment of various maladies. Objective of the study was to evaluate, one of the traditionally used drugs, Marselia minuta for its antimicrobial activity.
Methods: Extracts of M. minuta was prepared using successive solvent extraction method and the antibacterial activity of thus prepared petroleum ether, chloroform, ethyl acetate, alcohol and aqueous extracts of M. minuta was performed using agar well diffusion method. Four bacterial strains were used to assess the antimicrobial properties of the plant extracts viz Escherichia coli, Streptococcus pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus.
Results: All the extracts showed activity towards the tested microorganisms except petroleum ether extract. Ethyl acetate extract was more active towards S. aureus and P. aeruginosa. Alcohol and chloroform extract was active toward E. coli and P. aeruginosa. Aqueous extract was active against E. coli and S. aureus. minimum inhibitory concentration were calculated for each extract and the Significant activity was observed for ethyl acetate extract (E. coli-11.33 mm, S. aeruginosa-16.0 mm) and alcoholic fractions (E. coli-15.33 mm and P. aeruginosa-10.66 mm) for concentration 500 mcg/ml.
Conclusion: Thus, the result in the present experiment ensures the traditional use of M. minuta as antibacterial agent. Further, the most active extracts can be subjected for isolation of the therapeutic antimicrobials and its formulation which will be relatively safer than synthetic medicines and offering profound therapeutic benefits at the more affordable price.
Das K, Tiwari RKS, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: current methods and future trends. J Medicinal Plant Res 2010;4(2):104â€“11.
Dharamuir H. Bioactive Madicinal plants. Gene tech books: New Delhi; 2002;56:57-75.
Iwu MM, Duncan AR, Okunji CO. New Antimicrobials of Plant Origin. Perspectives on new crops and new uses. ASHS Press: Alaxandria; 1999. p. 457â€“62.
Tiwari OP, Subhrata K, Bhattamisra, Pushpendra K, tripathi, Paras N. Anti aggressive activity of standardized extract of Marsilea minuta Linn. In rodent models of aggression. Bio Sci Trends 2010;4(4):190-4.
Pulok KM. Quality control of herbal drugs. 2nd ed. New Delhi: Business Horizons; 2007.
Mohana DC, Satish S, Raveesha KA. Antibacterial evaluation of some plant extracts against some human pathogenic bacteria. Adv Biol Res 2008;2:49â€“55.
Bonev B, Hooper J, Parisot J. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrobial Chemotherapy 2008;61(6):1295â€“301.
Spellberg B, Powers JH, brass EP, Miller LG, Edwards JR. trends in antimicrobial drug development: implications for the future. Clin Infect Dis 2004;38:1279-86.
Yuan Q, Wang J, Ruan J. Screening for bioactive compounds from adiantum capillusâ€“ veneris L. J Chem Soc Pak 2012;34(1):207-16.
Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). Afr J Pharm Pharmacol 2009;3(2):58â€“62.
Neeraj M, Behal KK. Antimicrobial activity of some species against selected microbes. Int J Pharm Pharm Sci 2010;2(3):187-96.