EFFECT OF SUGNIL, A TRADITIONAL ANTIDIABETIC HERBAL FORMULATION ON THE EXPRESSION OF TRANSCRIPTION FACTORS IN STREPTOZOTOCIN DIABETIC RATS.
Keywords:Transcription factors, Nil, SREPB-1, SREPB-2
Objective: A number of large randomized clinical trials demonstrated that lipid lowering successfully reduced cardiovascular events and death in patients with diabetes. Recent studies have reported that multiple transcription factors are involved in regulation of lipid metabolism in diabetic subjects. Numerous plant extracts and formulations have been shown to regulate the expression of transcription factors which in turn normalize lipid abnormalities in diabetic patients. In this series, sugnil, a traditional anti-diabetic polyherbal formulation was assessed for its regulatory effect on the expression of transcription factors in lipid metabolism of streptozotocin induced diabetic rats.
Methods: The mRNA expressions of fatty acid synthesis related genes such as SREPB-1 and SREPB-2 and fatty acid decomposition related gene PPARÎ± were measured by real-time RT-PCR using an appropriate primers and thermal cycler conditions.
Results: Oral administration of sugnil to diabetic rats for 42 consecutive days effectively normalized the expression of these transcription factors to near normal as in control rats. Expression of PPAR-Î± was found to be up-regulated that leads to diminished expression of SREBP-2 in the liver of diabetic rats treated with sugnil. Treatment with sugnil also down regulated the SREBP-1 expression in the kidney of diabetic rats.
Conclusion: The molecular mechanisms of action of sugnil in the regulation the lipid profile in diabetic rats might be normalizing the expression of these transcription factors.
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