EFFECT OF SOLVENT EXTRACTION SYSTEM ON THE ANTIOXIDANT ACTIVITIES OF ALGAE
Keywords:Algae, Different solvent extracts, Antioxidant activity
Objective: The present study aimed to determine the antioxidant activities of five algae including Oedogonium globossum, Pithophora oedogonia, Rhizoclonium hieroglyphicum, Scytonema ocellatum and Spirogyra communis and the effect of different solvent (benzene, chloroform, acetone and methanol) used for the extraction of active components from algae.
Methods: The five algae were extracted with four different solvents e. g benzene, chloroform, acetone and methanol. The Folin-Ciocalteu procedure was used to estimate the amount of total phenolic content in different extracts of algae. Total flavonoid content was estimated by aluminium chloride colorimetric method. The DPPH radical scavenging assay was used to find out the antioxidant activities of algae under investigation.
Results: The total phenolic content varied from 9.80Â±0.29 to 44.44Â±2.26 mg/g, 7.26Â±0.08 to 21.30Â±0.17 mg/g, 9.82Â±0.12 to 50.14Â±0.99 mg/g and 11.51Â±0.17 to 54.02Â±0.58 mg/g dry material in the benzene, chloroform, acetone and methanol extracts of the algae respectively. The flavonoid content was found to be the highest in the acetone extract of Scytonema ocellatum (593.81Â±2.69 mg/g) and flavonol content was found to be the highest in the chloroform extract of Scytonema ocellatum (448.22 Â± 4.36 mg/g) whereas least amount of flavonoid present in the benzene extract of Oedogonium globossum 8.91Â±0.37 and least amount of flavonol was present in the chloroform extract of Spirogyra communis (13.54Â±1.07 mg/g). The highest DPPH radical scavenging was observed in the methanol extract of Scytonema ocellatum with IC50 = 0.24Â±0.003 mg dry material. The reducing power of the extracts of the algae was also evaluated as mg AAE (ascorbic acid equivalent)/g dry material. Chloroform extract of Rhizoclonium hieroglyphicum showed the highest reducing power 185.56Â±2.00 mg AAE/g dry materials.
Conclusion: Thus it could be suggested that these algae can be utilized as natural antioxidant.
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