PHYTOCHEMICALS, ANTIOXIDANT AND ANTIPROLIFERATIVE PROPERTIES OF FIVE MOSS SPECIES FROM SABAH, MALAYSIA
Keywords:Phytochemical contents, Antioxidant activity, Antiproliferative activity, Mosses
Objective: This study was conducted to investigate the phytochemical contents, the antioxidant and antiproliferative properties of 80% methanol, ethanol and aqueous extracts of Sphagnumcuspidatumsubsp. subrecurvum, Sphagnumcuspidatulum, Sphagnumjunghuhniannum, Pogonatumcirratumsubsp. fuscatum and Pogonatumcirratumsubsp. macrophyllum.
Methods: The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. The antioxidant properties were evaluated by three different assays, namely, ferric reducing/antioxidant power (FRAP), ABTS and DPPH free radical scavenging assays. MTT assay was used to study the antiproliferative properties against selected cancer cell lines.
Results: The results showed that the aqueous and ethanol extracts of S. cuspidatumsubsp. subrecurvumhas the highest total phenolic and total flavonoid content with the values of 5.42 Â± 1.95 mg gallic acid equivalent per g of dry sample and 2.12 Â± 0.02 mg catechin equivalent per g of dry sample, respectively. The 80% methanol extracts of S. junghuhnianum has the lowest total phenolic and total flavonoid content with the values of 0.80 Â± 0.12 mg gallic acid equivalent per g of dry sample and 0.03 Â± 0.02 mg catechin equivalent per g of dry sample, respectively. None of the extracts displayed IC50 value(concentration that inhibits 50% of free radical) at concentration tested. The 80% methanol extracts ofP. cirratum subsp. fuscatuminduced antiproliferative activityagainst CaOV3 (ovarian carcinoma) cell line whereas S. cuspidatulum induced antiproliferative activity againstHepG2 (liver cancer) cell line. None of the extracts induce antiproliferative activity against MDA-MB-231 (non-hormone dependent breast cancer) cell line.
Conclusion: Results obtained indicated that the selected mosses contained considerable amount of phenolics and flavonoids which contribute to antioxidant properties. P. cirratumsubsp. fuscastumand S. cuspidatulum have the potential to be used in pharmaceutical industry due to the ability of these two species to induceantiproliferative activity against CaOV3and HepG2 cancer cell lines.
Akinmoladun AC, Ibukun EO, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimumgratissimum. Sci Res Essays 2007;2(5):163-6.
Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agricul Food Chem 2001;49:5165-70.
Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiberofficinale Roscoe). Molecules 2010;15:4324-33.
Fu L, Xu BT, Xu XR, Gan RY, Zhang Y, Xia EQ, et al. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem 2011;129:345-50.
Pejin B, BogdanoviÄ‡-Pristov J. ABTS cation scavenging activity and total phenolic content of three moss species. Hem Ind 2012;66(5):723-6.
Asakawa Y. Biologically active compounds from bryophytes. Pure Appl Chem 2007;79(4):557-80.
Asakawa Y. Liverworts-Potential source of medicinal compounds. Medici Aromatic Plants 2012;1:3.
Manoj GS, Murugan K. Phenolic profiles, antimicrobial and antioxidant potentiality of methanolic extract of a liverwort, Plagiochiabeddomei Steph. Indian J Nat Prod Resour 2012;3(2):173-83.
Kumar P, Chaudhary BL. Antibacterial activity of moss Endotodonmyurus (Hook) Hamp. against some pathogenic bacteria. Bioscan 2010;5(4):605-8.
Wang D, Zhu RL, Qu L. Antibacterial activity in extracts of Cylindrocolearecurvifolia (Cephaloziellaceae, Marchantiophyta) and Pleuroziasubinflata (Pleuroziaceae, Marchantiophyta) Cryptogamie. Bryologie 2006;27(3):343-8.
Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agricultural Food Chem 1998;46(10):4113-7.
Zhishen J, Mencheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals. Food Chem 1999;64:555-9.
Mensor LI, Menezes FS, Leitao GG, Reis AS, Santos TD, Coube CS, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotheraphy Res 2001;15:127-30.
Benzie IF, Strain JJ. The ferric reducing ability of plasma(FRAP) as a measure of â€˜â€˜antioxidant powerâ€™â€™: The FRAP Assay. Anal Biochem 1996;239:70-6.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cationdecolorisation assay. Free Radical Bio Med 1999;26:231-1237.
Mossman BT, Light W, Wei ET. Asbestos: mechanisms of toxicity and carcinogenicity in the respiratory tract. Ann Rev Pharmacol Toxicol 1983;23:595-615.
TomsoneL, Kruma Z, Galoburda R. Comparison of different solvents and extraction methods for isolation of phenolic compounds from horseradish roots (Armoraciarusticana). World Academy Sci Eng Technol 2012;64:903-8.
Alam S, Mohiuddin M, Swamy KL, Baig A, Reddy MV, Gupta RK, Diwakar S. Evaluation of antiulcer and antioxidant activity of polyherbal formulation in wistar rats. Int J Pharm Phytopharmacol 2013;2(6):400-6.
Cheung LM, Cheung PCK, Ooi VEC. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 2003;81:249-55.
Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant properties of the methanol extracts of the leaves and stems of Celtisafricana. Rec Nat Prod 2009;3(1):23-31.
Bhattarai HD, Paudel B, Lee HS, Lee YK, Yim JH. Antioxidant activity of Sanioniauncinata, a polar moss species from King George Island, Antartica. Phytotherapy Res 2008;22:1635-9.
Chobot V, Kubicova L, Nabbout S, Jahodar L, Hadacek F. Evaluation of antioxidant activity of some common mosses. ZeitschriftfÃ¼r Naturforschung. C A J Biosci 2008;63(11):476.
Yang JH, Lin HC, Mau JL. Antioxidant properties of several commercial mushrooms. Food Chem 2002;77:229-35.
Wang M, Li J, Rangarajan M, Shao Y, LaVoie EJ, Huang TC, Ho CT. Antioxidative phenolic compounds from sage (Salvia officinalis). J Agricultural Food Chem 1998;46(12):4869-73.
Dalar A, TÃ¼rker M, KonczakI. Antioxidant capacity and phenolic constituents of Malvaneglecta Wallr. And Plantagolanceolata L. from Eastern Anatolia Region of Turkey. J Herbal Medici 2012;2:42-51.