REACTIVE OXYGEN AND NITROGEN SPECIES SCAVENGING AND ANTICANCER POTENTIAL OF CISSUS QUADRANGULARIS L. AGAINST EAC CELL LINE
Keywords:
Cissus quadranqularis L, Antioxidant, Phytochemicals, EAC cell line, CancerAbstract
Objective: To investigate the in vitro antioxidant and anticancer activity of chloroform and ethanol extracts of Cissus quadranqularis L. leaves.
Methods: Phytochemicals were analysed by using standard methods. In Vitro antioxidant studies were carried out for the chloroform and ethanol extracts of the Cissus quadranqularis L using various free radical models such a DPPH, Reducing power assay, Nitric oxide, Hydrogen peroxide, Superoxide scavenging and ABTS. In vitro cytotoxic assay such as trypan blue dye exclusion and MTT assays were carried out both ethanol and chloroform extract against EAC cell line.
Results: The preliminary phytochemical screening of Chloroform extract contains alkaloids, sugar, quinines, steroids and tannins. Ethanol extract showed the presence of alkaloids, coumarin, quinines, steroids and sugar. The result revealed that the ethanolic extracts have significant antioxidant potential than chloroform extract. The result revealed that the ethanol extracts of Cissus quadranqularis L. showed pronounced activity against the tested cell line.
Conclusion: The result of the present study concluded that the ethanolic extracts of Cissus quadranqularis L. have significant antioxidant and anticancer activity then the chloroform extract. The potential antioxidant and anticancer activity of Cissus quadranqularis L. might be due to the presence of phytochemicals.
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