EVALUATION OF IN VITRO ANTICANCER POTENTIAL OF ETHANOLIC EXTRACT AND ITS DIFFERENT FRACTIONS OF CAESALPINIA BONDUC (L) ROXB SEEDS
Keywords:Caesalpinia bonduc, Anticancer, In vitro, In silico
Objective: The present study aims to evaluate the anticancer potential of ethanolic extract and its different fractions of Caesalpinia bonduc seeds against Ehrlich Ascites Carcinoma (EAC) cell lines.
Methods: Ethanolic extract and its fractions were subjected to preliminary phytochemical screening and anticancer activity by using in vitro anticancer assay. Chemical constituents of petroleum ether fraction of C. bonduc seeds were identified by using GC-MS and the active compounds were subjected to in silico studies.
Results: In vitro anticancer assay showed that the petroleum ether fraction of ethanolic extract of Caesalpinia bonduc seeds has potential anticancer activity.
Conclusions: Petroleum ether fraction of ethanolic extract of Caesalpinia bonduc seeds has significant anticancer activity. Further in-depth studies, could result in the development of a good anticancer agent from the seeds of Caesalpinia bonduc.
Komal moon, SS Khadabadi, UA Deokate, SL Deore. Govt College of Pharmacy, Caesalpinia bonducella. Over view 2010.
Vibha Singh, Pramod KRaghav. Dept of biotechnology and Allied Sciences. Int J of Medical Aroma Plants 2012.
Asolkar LV, Kakkar KK, Chakre OJ. Second Suppl. To Glossary of Indian Medicinal Plants with Active Principles, PID-CSIR;New Delhi:1992. p.150.
White R. Legume web [Internet]. version 10.01 Cardiff (UK):Cardiff University, School of Computer Sciences. c 2005 â€“ [Revised 2005 Nov 1;cited 2008 Sep 23]. Available from:http://www. ildis. org/Legumeweb.
WHO. in Progress Report by the Director General, Docum. J World Health Organization Geneva 1991.
Razia Sultana, Rubina Saleem, Nighat Sultana, Farhana Afshan, Tahsin Gulzar. Characterization of the Composition of Caesalpinia bonducella. Seed Grown in Temperate Regions of Pakistan J Am Oil Chem Soc 2012;89:1021â€“27.
Altaf ALI, N Venkat Rao, MD Shalam, T Shivaraj Gouda, SM Shantakumar. Anticonvulsive Effect of Seed Extract of Caesalpinia bonducella (Roxb.). Iranian J of Pharmacology and Therapeutics 2009;1735-2657/09/82-51-55.
Giwa OE, Seyifunmi OE, Adewumi BL, Adebote VT, Aladejimokun AO. Screening of Antimicrobial Ethanolic Extract of Peristrophe bicalyculata. J Ethnobotanical Leaflets 2010;14:766-73.
Gothoskar SV, Ranadive KJ. Anticancer screening of SAN-AB, An extract of marking nut Semicarpus anacardium. Indian J Exp Biol 1971;9:372-5.
Scudiero DA, Shoemaker RH, Paul KD. Evaluation of soluble terazolium formazan assay for cell growth and drug sensitivity in clusters using human and other tumor cell lines. J Cancer Res 1988;48:4827-33.