ISOLATION AND SCREENING OF MARINE MICROALGAE CHLORELLA SP. _PR1 FOR ANTICANCER ACTIVITY
Keywords:Chlorella sp _PR1, Anticancer, FACS, IC50 value
Objective: The objective of the present study includes isolation, characterization and screening of anticancerous activity against B16F10 cell line using isolated marine microalgae Chlorella sp._PR1.
Methods: In this study, marine microalgae Chlorella sp._PR1 isolated and cultured using f/2 medium and anticancerous activity was assayed using MTT assay.
Results: The DMSO extract of Chlorella sp._PR1 was exhibit anticancerous activity against murine melanoma B16F10 cell line. The extract exhibit reduction of cell viability up to 56% with 2Âµg/ml concentration. IC50 were calculated and was found that Chlorella sp._PR1 need 5.5 Î¼g/ml of the compounds to reduce the murine melanoma B16F10 cell viability by 50%. Fluorescence activated cell sorting (FACS) analysis revealed that Chlorella sp._PR1 extract (8 Âµg/ml) brought significant inhibition (p<0.01) of the G0-G1 and the S phase. The extract did not seem to affect the G2-M phase.
Conclusion: DMSO extract of Chlorella sp._PR1 (5.5 Âµg/ml) was found to be potent against murine melanoma B16F10 cell line.
Murugan K, Iyer VV. Antioxidant and antiproliferative activities of marine algae, Gracilaria edulis and Enteromorpha lingulata, from Chennai coast. Int J Cancer Res 2012;8:15â€“26.
Harada H, Noro T, Kamei Y. Selective antitumor activity in vitro from marine algae from Japan coast. Biol Pharm Bull 1997;20:541-6.
Xu N, Fan X, Yan XJ, Li X, Niu R, Tseng CK. Antibacterial bromophenols from the marine red alga Rhodomela confervoides. Phytochem 2003;62:1221-4.
Li XC, Jacob MR, Ding Y, Agarwal AK, Smillie TJ, Khan SI, et al. Capisterones A and B, which enhance fluconazole activity in Saccharomyces cerevisiae, from the marine green alga, Penicillus capitatus. J Nat Prod 2006;69:542-6.
Matsuhiro B, Conte AF, Damonte EB, Kolender AA, Matulewicz MC, Mejias EG, et al. Structural analysis and antiviral activity of a sulfated galactan from the red seaweed Schizymenia binderi (Gigartinales Rhodophyta). Carbohydr Res 2005;340:2392-402.
Wijesekara I, Pangestuti R, Kim S. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohyd Polym 2010;84:14â€“21.
Holdt SL, Kraan S. Bioactive compounds in seaweed: functional food applications and legislation. J Appl Phycol 2011;23:543-97.
Song L, Ren S, Yu R, Yan C, Li T, Zhao Y. Purification, Characterization and in vitro anti-tumor activity of proteins from arca subcrenata lischke. Marine Drugs 2008;6:418-30.
Folmer F, Japars M, Dictato M, Diederich M. Photosynthetic marine organisms as a source of anticancer compounds. Phytochem Rev 2010;9:557â€“79.
Mohamed S, Hasim SN, Rahman HA. Seaweeds: a sustainable functional food for complementary and alternative therapy. Trends Food Sci Technol 2012;23:83â€“96.
Guillard RRL. Culture of Phytoplankton for feeding marine invertebrates. In Culture of Marine Invertebrate Animals. Edited by Smith WL, Chanley MH. New York, USA: Plenum Press; 1975. p. 29â€“60.
Samarakoon KW, Ko JY, Shah MR, Lee JH, Kang MC, Nam Ko, et al. In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae. Algae 2013;28:111-19.
Andrianasolo EH, Haramaty L, Vardi A, White E, Lutz R, Falkowski P. Apoptosis-inducing galactolipids from a cultured marine diatom, Phaeodactylim tricornutum. Indian J Nat Prod 2008;71:1197-201.