SUPPRESSION OF N-NITROSODIETHYLAMINE INDUCED OXIDATIVE RENAL TOXICITY BY SULPHATEDPOLYSACCHARIDE AND AQUEOUS EXTRACT OF ULVA LACTUCA IN RATS
Keywords:
Ulva lactuca, Renal toxicity, N-nitrosodiethylamine, Antioxidant enzymes, ApoptosisAbstract
Objective: The ancient tradition habits of consuming seaweeds in Asian and Middle East countries have been made a key part of their diet, and as an antioxidant, sulphated polysaccharides have made a large number of researches as one of the ocean's greatest treasures. Therefore, the scope of the present study was conducted to investigate the chemopreventive actions of sulphated polysaccharides and aqueous extract of Ulva lactuca on N-nitrosodiethylamine (NDEA) promoted by phenobarbital (PB) induced renal toxicity in male albino rats.
Methods: Adult male albino rats were divided into four groups. Group B received a single dose of NDEA intra peritonealy (200 mg/kg body weight) for 2, 12 and 24 weeks to induce renal toxicity. Groups (C& D) simultaneously received (50 mg/kg body weight) sulphated polysaccharides and aqueous extract of U. lactuca, respectively by oral gavage. Further, the B, C& D groups received PB (0.05%) in drinking water after two weeks of NDEA administration along the entire period of study. Saline (0.9%) treated control group (A) was also built-in. Several In vivo enzymatic antioxidant defense system like superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione-s-transferase (GST), myeloperoxidase (MPO), and non-enzymatic system like reduced glutathione (GSH) were evaluated to determine the reno-protective and antioxidant activity of U. lactuca extracts. The oxidative stress markers like lipid peroxidation (LPO) and nitric oxide (NOx) were also investigated as the products of NDEA metabolism.
Results: NDEA followed by PB provoked renal and nephrotoxicity was evident from the decreased activity of almost antioxidant enzymes (SOD, CAT, GST, GR and MPO) and increased oxidants (LPO & NOx) in renal tissues. A significant rise in the levels of malondialdehyde (MDA) throughout the entire period of study along with the striking decline of the above enzymic and non-enzymic antioxidants marks the severity of oxidative stress in NDEA-induced rats. However, concomitant treatment with sulphated polysaccharides restored the above antioxidant enzyme levels in the kidneys near to normal better than the aqueous extract, and exhibited a significant dose dependent protective effect against NDEA induced nephrotoxicity, which might be attributed to the antioxidant properties of the extracts.
Conclusion: The present study provides evidence, for the first time, that sulphated polysaccharides exert a chemoreceptive significant effect on NDEA-initiated nephrotoxicity through induction of apoptosis. In addition, the restoration effect of enzymic and non-enzymic antioxidant to their normal levels. The study justified the ethno-medicinal use of sulphated polysaccharide extract for protection against renal toxicity.
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