EVALUATION OF TERATOGENICITY EFFECTS OF ETHANOLIC EXTRACTS OF BINAHONG LEAVES (ANREDERA CORDIFOLIA (TEN) STEENIS) IN WISTAR RAT
Keywords:
Binahong, Anredera cordifolia (Ten) Steenis, Teratogenic effect, TrimethoprimAbstract
Objective: The aim of this study was to evaluate teratogenicity effect of ethanolic extract of binahong (Anredera cordifolia (Ten.) Steenis) leaves in Wistar rats.
Methods: The teratogenicity test were done in 5 groups of rat i. e. control group, trimethoprim at a dose of 360 mg/kg bw, ethanolic extract of binahong at doses of 100, 400, and 1000 mg/kg bw. Test substance was given orally on day 6 to day 15 of rats pregnancy. At day 20 of gestation, laparoctomy was performed to retrieve the fetuses. Evaluation was done towards the fetus skeletal, organ and fetal weight.
Results: Teratogenicity test results showed that there was the ungrowth fetus observed in all groups with the highest incident in trimethoprim group (39.5%). Average of fetus body weight of trimethoprim, control, binahong extract at doses of 100, 400 and 1000 mg/kg bw group was 2.32 ± 0.66 g, 3.58 ± 0.24 g, 3.64 ± 0.26 g, 3.67 ± 0.25 g and 3.93 ± 0.61 g, respectively. Skeletal evaluation showed that there was malformation of sacral vertebrae (65.2%), convulated costal stucture (82.6%), palate (33.3%), head cavity (88.9%), nasal cavity (5.6%), eye cavity (5.6%), ear (5.6%) and liver (43.1%) which were observed only in trimethoprim group.
Conclusion: Ethanolic extract of binahong at doses of 100, 400 and 1000 mg/kg bw in rat did not have the teratogenic effect.
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