FACTORS INFLUENCE ON GROWTH, DON AND NIV PRODUCTION BY TWO SPECIES OF FUSARIUM ISOLATED FROM FINGER MILLETS [ELEUSINE CORACANA L.]

Authors

  • P. Shilpa Department of Microbiology, Kakatiya University, Warangal, Telangana, India
  • V. Koteswara Rao Department of Microbiology, Kakatiya University, Warangal, Telangana, India
  • S. Girisham Department of Microbiology, Kakatiya University, Warangal, Telangana, India
  • S. M. Reddy Department of Microbiology, Kakatiya University, Warangal, Telangana, India

Keywords:

Finger millets, F aethiopicum, F culmorum, Synthetic media, pH, Temperature, Microbial nutrients, DON, NIV, HPLC

Abstract

Objective: The present investigations (In vitro) on an influence of different synthetic and food based [flour] media, pH, temperature and microbial nutrients on growth, Deoxynivalenol [DON] and Nivalenol [NIV] production by Fusarium aethiopicum and F. culmorum was carried out.

Methods: Fusarium species associated with the finger millets were isolated and identified phenotypically and further confirmed by molecular methods by Polymerase Chain Reaction [PCR]. Monosporic seven day old F. aethiopicum and F. culmorum were grown in CYA broth and incubated at 27±2°C on rotary shaker for 21 days at 120 rpm. At the end of 21 day incubation period, cultures were harvested for determination of fungal biomass. The resultant culture filtrates were extracted twice with ethyl acetate and concentrated to get and final concentration of 1 ml in methanol and employed for RP-HPLC analysis for detection of DON and NIV production.

Results: Finger millets flour medium induced the highest amount of mycelial growth, DON and NIV production by F. aethiopicum. However, F. culmorum achieved highest amount of hyphal growth, DON and NIV production under the influence of yeast extract sucrose [YES] medium. Maize flour medium, rice flour medium and sorghum flour medium was next preffered substrates. Optimum pH of 5.5-6.5 and temperature of 20-35°C for growth, DON and NIV production was observed. Both the species of Fusarium failed to grow and produce toxins at pH 2.5-3.5 and temperature of 40°C. Yeast extract was most favorable for maximum DON and NIV production, which increased with an increase in its concentration. On the other hand, malt extract and beef extract induced good growth and mycotoxin production at comparatively higher concentration in both the species of Fusarium under study.

Conclusion: Comparatively food based media were the better substrates than synthetic media for both growth and DOIN and NIV production by two species of Fusarium under investigation. A positive correlation coefficient [r] on growth [0.458], DON [0.744] and NIV [0.882] was recorded among the media and both the species of Fusarium.

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References

ICRISAT/FAO. The World Sorghum and Millet Economies: Facts, Trends and Outlook. ICRISAT/FAO: Patancheru, India; 1996. p. 1-2, 31-40.

Obilana AB, Manyasa E. Millets. In: Pseudo cereals and less common cereals. Grain properties and utilization potential PS Belton, JRN Taylor eds. Springer-Verlag. Berlin; 2002. p. 176-217.

Sen S, Dutta SK. Evaluation of anti-cancer potential of ragi bifunctional inhibitor [RBI] from Elusine coracana on human chronic myeloid leukemia cells. Eur J Plant Sci Biotechnol 2012;6:103-8.

Ramana MV, Balakrishna K, Murali HS, Batra HV. Multiplex PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in rice and fingermillet collected from southern India. J Sci Food Agric 2011;91:1666–73 .

Shilpa P, Girisham S, Reddy SM. Elaboration of mycotoxins by seed-borne fungi of finger millet [Eleusine coracana L]. Int J Biotech Molecularbiol Res 2010;1:62-4.

Sugiura Y, Watanabe Y, Tanaka T, Yamamoto S, Ueno Y. Occurrence of Gibberella zeae strains that produce both nivalenol and deoxynivalenol. Appl Environ Microbiol 1990;56:3047-51.

Birzele B, Prange A, Kramer J. Deoxynivalenol and ochratoxin A in German wheat and changes of levels in relation to storage parameters. Food Addit Contam 2000;17:1035–9.

Schollenberger M, Muller HM, Ernst K, Sondermann S, Liebscher M, Schlecker C, et al. Occurrence and distribution of 13 trichothecene toxins in naturally contaminated maize plants in germany. Toxins Basel 2012;4:778–87.

Saleh AA, Esele JP, Logrieco A, Ritieni A, Leslie JF. Fusarium verticillioides from finger millet in Uganda. Food Addit Contamin Part A 2012;29:1762-9.

Pestka JJ, Bondy GS. Immunotoxic effects of mycotoxins. In: Miller JD and Trenholm HL. [Eds.] Mycotoxins in grain. Compounds other than aflatoxins. Eagan Press, Pt. Paul [MN] USA; 1994. p. 339-58.

Eriksen GS, Alexander J. Fusarium toxins in cereal risk assessment. Copenhagen, Nordic Council of Ministers Eds; 1998. p. 146.

Lacey J. Natural occurrence of mycotoxins in growing and conserved forage crops. In: Smith JE, Henderson RE [eds]. Mycotoxins and animal foods. CRC Press, Boca Raton; 1991. p. 363–97.

Rao CY. Toxigenic fungi in the indoor environment. In: Spengler JD, Samset JM, McCarthy JS [eds] Indoor air quality handbook. McGraw-Hill, Washington, DC; 2001. p. 1-17.

International Seed Testing Association [ISTA]. Seed Sci Tech 1985;13:299-355.

Samson RA, Hoekstra, Van Oorschot CN. Introduction to food-borne fungi. Institute of the royal Netherlands, Academy of Arts and Science; 1984. p. 217-36.

Nelson PE, Toussoun TA, Marasas WF. Fusarium species: an illustrated manual for identification. The Pennsylvania State University Press, University Park; 1983. p. 193.

Shilpa P, Koteswara Rao V, Girisham S, Reddy SM. Natural incidence of fusarial mycotoxins in Finger millet [Eleusine coracana L.] of Andhra Pradesh, India. Asiatic J Biotechnol Resor 2011;2:392-402.

Payne GA. Ear and kernel rots. In: White DG [ed] Compendium of corn diseases. The American Phytopathol Society Press, St. Paul; 1999. p. 44–7.

Narasimha Rao K, Vijaypal Reddy B, Girisham S, Reddy SM. Factors influencing fumonisins [B1] production by Fusarium moniliforme. Indian. J Sci Technol 2010;3:213–5.

Koteswara Rao V, Ramana MV, Girisham S, Reddy SM. Culture media and factors influencing ochratoxin a production by two species of penicillium isolated from poultry feeds. Natl Acad Sci Lett 2013;36:101-10.

Wheeler KA, Hurdman BA, Pitt JF. Influence of pH on the growth of some toxigenic species of Aspergillus, Penicillium and Fusarium spp. Int J Food Microbiol 1991;12:141-50.

Surekha M, Reddy SM. Influence of temperature and pH on the production of penitrem B by P. aurantiogriseum. Nat Acad Sci Lett 1991a; 15:463-5.

Mitchell D, Parra R, Aldred D, Magan N. Water and temperature relations of growth and ochratoxin a production by Aspergillus carbonarius strains from grapes in Europe and Israel. J Applied Microbiol 2004;97:439–45.

Cabanes FJ, Accensi F, Bragulat MR, Abarca ML, Castella G, Minguez S, et al. What is the source of Ochratoxin A in wine. Int Food Microbiol 2002;79:213–5.

Published

01-11-2014

How to Cite

Shilpa, P., V. K. Rao, S. Girisham, and S. M. Reddy. “FACTORS INFLUENCE ON GROWTH, DON AND NIV PRODUCTION BY TWO SPECIES OF FUSARIUM ISOLATED FROM FINGER MILLETS [ELEUSINE CORACANA L.]”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 11, Nov. 2014, pp. 312-7, https://journals.innovareacademics.in/index.php/ijpps/article/view/3170.

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