BIOLOGICAL SCREENING OF ARAUCARIA COLUMNARIS

Authors

  • Muhammad Shahzad Aslam BAHAUDDIN ZAKARIYA UNIVERSITY, MULTAN
  • Bashir A. Choudhary BAHAUDDIN ZAKARIYA UNIVERSITY, MULTAN
  • Abdul Subhan Ijaz BAHAUDDIN ZAKARIYA UNIVERSITY, MULTAN
  • Muhammad Uzair BAHAUDDIN ZAKARIYA UNIVERSITY, MULTAN
  • Muhammad Syarhabil Ahmad School of Bioprocess Engineering, University Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, 02600 Arau, Perlis, Malaysia.
  • Awang Soh Mamat School of Bioprocess Engineering, University Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, 02600 Arau, Perlis, Malaysia.

Keywords:

Araucaria columnaris, Biological screening, Phytotoxicity, Weedicide research

Abstract

Objective: The present research is biological screening of aerial part of Araucaria columnaris (Araucariaceae). There were investigation for their antibacterial, antifungal, phytotoxic and cytotoxic activities of Dichloromethane and methanol extract.

Methods: Anti-bacterial, Anti-fungal, cytotoxicity and phytotoxicity activity was performed by Disc diffusion method, Agar tube dilution assay, Brine Shrimp Lethality bioassay and Lemna bioassay respectively.

Results: Dichloromethane and methanolic extract exhibited significant phytotoxicity against Lemna minor having Paraquat as standard drug and incubation condition (28±1 °C). None of extracts presented any significant antibacterial and cytotoxic activity having Imipenum and Etoposide as standard drug respectively. Both extract had non-significant antifungal activity but it has been noted that MeOH and DCM extract of Araucaria columnaris showed 10% and 20% inhibition with linear growth at 90 mm and 80 mm respectively, when compared with control; against Fusarium solani and Aspergillus flavus respectively.

Conclusion: Araucaria columnaris exhibited significant phytotoxicity bioassay. The phytotoxicty assay is a useful primary screen for weedicide research. Synthetic weedicides are expensive, toxic and non-specific. This study will help to discover the phytotoxic constituents of the plant by isolation, purification and structure elucidation to find out as effective herbicidal.

 

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References

Nasir E, Ali SI. Flora of Pakistan. Pakistan V; 1986. p. 181.

Aslam MS, Choudhary BA, Uzair M, Ijaz AS. Phytochemical and ethno-pharmacological review of the genus araucaria–review. Tropical J Pharm Res 2013;12(4):651-9.

Atta-ur-Rhman, Choudhary MI, Thomsen WJ. Bioassay technique for drug development. Harwood Academic Publishers; 2001. p. 16-7, 23-4, 65-6.

McLaughlin JL. Crown gall tumors on potato discs and brine shrimp lethality: two single bioassays for plant screening and fraction. In: Hostetsmann K. ed. Methods in Plant Biochemistry. Academic Press, London; 1991. p. 1-31.

Published

01-04-2015

How to Cite

Aslam, M. S., B. A. Choudhary, A. S. Ijaz, M. Uzair, M. S. Ahmad, and A. S. Mamat. “BIOLOGICAL SCREENING OF ARAUCARIA COLUMNARIS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 4, Apr. 2015, pp. 313-5, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/3263.

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Original Article(s)