EVALUATION OF CYTOTOXICITY, OXIDATIVE STRESS, NUCLEAR CHANGES AND PRO-INFLAMMATORY CYTOKINES INDUCED BY MONOCROTOPHOS IN HUMAN KERATINOCYTE CELLS IN VITRO
Keywords:Cytokine, Pesticide, Cell viability, HaCaT cells, Toxicity
Objective: This study was explored to identify the toxicological behaviour of monocrotophos against human keratinocyte cells (HaCaT cells) Methods: In this study HaCaT cell line was used to identify the inflammatory effect of monocrotophos on cell viability, nitric oxide secretion (NO), lactate dehydrogenase release (LDH), malondialdehyde release (MDA), nuclear changes, reactive oxygen species generation and cytokine expression
Results: From the in vitro cell viability study, it appears that the monocrotophos was toxic to HaCaT cells; IC50 value was 408.2 Âµg/mL during 24 h of incubation period. Significant increase in NO, LDH, MDA, nuclear changes, pro-inflammatory cytokine and ROS generation was observed compared with the control. 1/5th IC50 value treatment of HaCaT cells with monocrotophos resulted in 9.97, 8.78 and 9.45 times increase in TNF-Î±, IL-6 and IL-8 expression higher than the control.
Conclusion: This study gives perceptiveness about the toxicity of monocrotophos and provides wide acquaintance to restrict the use of monocrotophos.
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