IN VIVO TOXICITY STUDIES OF BIOSYNTHESIZED SILVER NANOPARTICLES USING BRASSICA OLERACEAE IN ZEBRA FISH MODEL
Keywords:
Red cabbage, Nanoparticles, Brassica oleraceae, Conventional method, Microwave assisted methodAbstract
Objective: Nanotechnology opens new applications in many fields including medicine, material science and various technologies. The aim of the current study is to synthesise nanoparticles of Brassica oleraceae by green chemistry approach which provides advancement over physical and chemical methods.
Methods: Silver nanoparticles of Brassica oleraceae were synthesized by conventional and microwave assisted methods. Further characterization was done using UV-Visible, FT IR, Scanning electron and Transmission electron microscopy. The synthesized silver nano particles of Brassica oleraceae were tested for cytotoxicity in Vero and Human epithelial carcinoma cells (HEp -2). The induction and characterization of apoptosis in treated cells were studied using Acridine orange and DAPI staining procedures. In vivo toxicity of silver nanoparticles of Brassica oleraceae was studied in the developmental stages of Zebra fish embryos. The antioxidant potential and level of oxidative stress induced by studying various enzymes in xenobiotic metabolism such as GST, GSH, GPx (inducers of xenobiotic metabolism) in Vero and HEp -2 cells.
Results: The nanoparticles of Brassica oleraceae were able to induce apoptosis in HEp-2 cells and as a potential inducer of Xenobiotic metabolism. Toxicity of the nanoparticles was assessed by studying the rapid diffusion of nanoparticles which is evidently observed in the developing embryos of Zebra fish.
Conclusion: The synthesized nanoparticles of Brassica oleraceae oleraceae were found to be stable and less toxic in normal cells when compared to cancer cells
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