EVALUATION OF IN VITRO ANTI-OXIDANT AND ANTI-ARTHRITIC ACTIVITY OF METHANOLIC EXTRACT OF MARINE GREEN ALGAE CAULERPA RACEMOSA
Keywords:Caulerpa racemosa, Anti-oxidant, Diclofenac sodium, Anti-arthritic activity, Protein denaturation
Objective: The present study was aimed to evaluate in vitro anti-oxidant activity and in vitro anti arthritic activity of methanolic extract of Caulerpa racemosa.
Methods: The in vitro anti oxidant activity was evaluated by using following method viz; DPPH scavenging activity, Nitric oxide scavenging activity, Total anti-oxidant activity, Determination of reducing power. The in vitro anti arthritic activity was evaluated by using protein denaturation method.
Results: Methanolic extract of Caulerpa racemosa showed a very good anti-radical activity in scavenging DPPH radical and nitric oxide radical with maximum % inhibition of 88.87Â±1.05% at 2000Î¼g/0.1 ml concentration and 80.49Â±1.43% at 2000Î¼g/ml respectively. Total anti-oxidant activity and reducing power of Caulerpa racemosa was found to be 32Î¼g equivalents of ascorbic acid at 500 Î¼g/ml and 88.80Â±0.98% at 2000Î¼g/0.05 ml respectively. The methanolic extract showed 49.33Â±0.597% of percentage inhibition at 1000Î¼g/0.05 ml by protein denaturation method.
Conclusion: From the results obtained, it can be concluded that the methanolic extract of Caulerpa racemosa possesses significant anti-oxidant and anti-arthritic activity. Further studies are required to signify the mechanism of action of these pharmacological activities and to mark them out for their significant pharmacological actions.
Heo SJ, EJ Park, KW Lee, YJ Jeon. Antioxidant activities of enzymatic extract from brown sea weeds. Bioresour Technol 2005;96:1613-23.
Heinz Lullmann, Albrecht Ziegler, Klaus Mohr. Color atlas of pharmacology. 2nd ed; 2000. p. 320.
Goodman, Gilman. Manual of Pharmacology and Therapeutics. McGraw-Hill companies; 2008. p. 54.
L Shravan Kumar N, Kishore G, Siva Kumar G, SindhuPriya ES. In-vitro anti-inflammatory and anti-arthritic activity of leaves of Physalis angulata. Int J Pharm Ind Res 2011;1(03):211-3.
Blois Ms. Antioxidant determination by the use of stable free radical. Nature 1958;181:1199-200.
Sanchez Moreno C, Larraurij A, Saura-calixto FA. Free radical scavenging capacity of selected of selected red rose and white wines. J Sci Food Agric 1990;79:1301-4.
R Lavanya, S Umamaheshwari, G Harish, J Bharath Raj, S Kamali, D Hemamalini, et al. Uma Maheshwara Reddy. In-vitro anti-oxidant studies of Anisomeles malabarica and Coldenia procumbene L. Res J Pharm Biol Chem Sci 2010;1(4):737-44.
Polterait O. Antioxidants and free radical scavengers of natural origin. Curr Org Chem 1997;1:415-40.
Repetto MG, Liesuy SF. Antioxidant properties of natural compounds used in popular medicine for gastric ulcer. Braz J Med Biol Res 2002;35:523-34.
Satoskar RS, Bhandarkar SD, Anipure SS. Pharmacology and pharmacotherapeutics, In, Pharmacotherapy of gout. 18th edi. Rheumatoid arthritis and osteoarthritis; 2003. p. 1022-7.
Richaupadhyay, Jitendra kumar chaurasia, Kavindra Nath Tiwari, Karuna Singh. Antioxidant property of aerial parts and root of phyllanthus fraternus webster, an important medicinal plant. Scientific World J 2014;2014:2-5.
MR Saha, SMR Hasan, R Akter, MM Hossain, MS Alam, MA Alam, MEH Mazumder. In vitro free radical scavenging activity of methanol extract of the Leaves of mimuso pselengi linn. Bangl J Vet Med 2008;6(2):197â€“202.
Palash Mandal, Tarun Kumar Mishra, Mitali Ghosal. Free radical scavenging activity and phytochemical analysis in the leaf and stem of Drymaria diandra Blume. Int J Integr Biol 2009;7(2):80-4.
Mediacano A, Alcaraz O, Arnao MB. Free radical scavenging activity of indolic compounds. Anal Bioanal Chem 2003;376:33-7.
Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japan J Nutr 1986;44(6):307â€“15.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.
Raghu KL, Ramesh CK, Srinivasa TR, Jamuna KS. Total antioxidant capacity in aqueous extracts of some common vegetables. Asian J Exp Biol Sci 2011;2(1):58-62.
S Banerjee, A Chanda, A Ghoshal, R Debnath, S Chakraborty, R Saha, et al. Nitric oxide scavenging activity study of ethanolic extracts of from two different areas of kolkata ixora coccinea. Asian J Exp Biol Sci 2011;2(4):595-9.
Abubakar B Aliyu, Mohammed A Ibrahim, Aliyu M Musa, Aisha O Musa, Joyce J Kiplimo, Adebayo O Oyewale. Free radical scavenging and total antioxidant capacity of root extracts of Anchomanes difformis Engl. (araceae). Acta Pol Pharm 2013;70(1):115-21.