EFFECT OF VARYING CONCENTRATION OF HERBAL EXTRACT OF NYCTANTHES ARBOR-TRISTIS LEAF ON SYNTHESIS OF SILVER NANOPARTICLES AND ITS EVALUATION
Keywords:
Silver nanoparticles, UV-vis, DLS, AFM, Antioxidant, Antibacterial potentialAbstract
Objective: Synthesis of silver nanoparticles (AgNPs) using N. arobor-tristis aqueous leaf extract with the aid of micro wave is being demonstrated here.
Methods: UV-visible spectrophotometer, dynamic light scattering (DLS), atomic force (AFM) and transmission electron microscopic (TEM) analysis was used to characterize AgNPs. Antioxidant activity was assessed using DPPH assay and antibacterial potential was measured using the well diffusion assay.
Results: AgNPs formation was completed after 120 sec of microwave exposure. The particles were crystalline and mostly spherical in shape. Antioxidant potential was proved through DPPH assay. AgNPs demonstrated significant antibacterial potential against both Gram negative (E. coli) and Gram positive (S. aureus).
Conclusion: The results show that AgNPs have suitable antioxidant & antibacterial activity and thus have potential to be employed as a therapeutic and/or cosmetic agent.
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