BIOTRANSFORMATION OF PROGESTERONE TO 17Α-HYDROXYPROGESTERONE BY USING PLANT CELL SUSPENSION CULTURE OF CATHARANTHUS ROSEUS
Keywords:
Catharanthus roseus, Apocynaceae, Cell suspension culture, Biotransformation, Progesterone, NilAbstract
Objective: The present investigation was aimed to perform biotransformation potential of Catharanthus roseus.
Methods: Cell suspension culture of C. roseus were prepared by using Murashige and Skoog medium. Biotransformation of an important bioactive progesterone (4-pregnane-3, 20-dione) was confirmed by cell suspension culture of Catharanthus roseus.
Results: Progesterone, which was used as precursor in cell suspension culture, was found to be bio transformed within three days into a new product through a regioselective hydroxylation process. The results confirmed that most of the progesterone added, was hydroxylated at its 17α-position leading to 17α-hydroxyprogesterone as a major biotransformation product after twelve days of incubation with 20% v/v cell density. The optimum biotransformation was achieved with the concentration of 100μg/ml progesterone at pH 5.5. Similarly, glucose was found as the most suitable source of carbon. The optimum incubation time was observed to be 12 days at 20% v/v cell density. Also higher progesterone biotransformation was observed using un immobilized cells as compared to immobilized cells.
Conclusion: The results of present work suggest that C. roseus can successfully biotransformed to the product similar to an original organic substrate, which is entirely synthetic. Such a biotechnological process could be of great interest for production of new chemical compounds like cortisone, hydroxycortisone and other important steroids.
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