• Fawkyeia A. El-hodairy Physiology Department, NODCAR, Giza, Egyp.


Bisphinol A, Streptozetocin, Pimpinella anisum, Neurotoxicity


Objective: Bisphenol A (BPA) was a polycarbonate plastic used in plastic containers, baby's bottle, water bottles, laptops, mobiles, and food canes, etc. It is associated with obesity and insulin resistance. The food and Drug Administration (FAD), The Environmental Protection Agency (EPA) and the Occupational Safety and Health Administration (OSHA) recommended more study on this material. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. Diabetes can cause many complications like cardiovascular risk, chronic renal failure, nephropathy etc. The present study was done to know how to decrease the toxic effect of BPA by Pimpinella anisum (Anise) on the physiological and neurological parameter on normal and diabetic rats induced by Streptozetocin (STZ).

Methods: Rats involved in this study were divided into eight groups: 1st control group and received citrate buffer, 2nd diabetic group treated with STZ (50mg/kg. b. wt), 3rd BPA group treated with BPA (30mg/kgbwt), 4th Anise group treated with anise oil. (3g/kgb. wt),5th STZ + BPA group combination between STZ and BPA with the same dose, 6th group treated with (STZ+Anise), 7th group treated with (STZ + Anise + BPA) and 8th group received Anise and BPA. All control and treatment were subsisted one month ago. Norepinephrine (NE), Dopamine DA and Serotonin (5HT) were determined in homogenate brain tissue by HPLC.

Results: The results showed the significant increase in NE, DA and 5HT in diabetic rats treated with (STZ), and in diabetic rats with BPA (STZ + BPA) in comparison with vehicle and diabetic (STZ) group. The results also showed a significant increase in glucose, triglyceride, cholesterol, ALT and AST. After treatment with (Anis), a significant decrease in NE, DOP and 5HT in diabetic rats treated with Anise (STZ + Anise) and diabetic rats treated with (Anise + BPA) in comparison with vehicle and diabetic (STZ) group. Thus, from this study,

Conclusion: It is concluded that, (BPA) has neurotoxic and this neurotoxicity was increased with diabetes and decreased by the treatment with anise.


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Bown, Deni. The Herb Society of America New Encyclopedia of Herbs & Their Uses. New York: DK, cardiovascular disease in men and women with diabetes compared with medicinal plants; 2001.

Mata AT, Proenc C, Ferreira AR, Serralheiro MLM, Nogueira JMF, Araujo MEM. Antioxidant and anti acetylcholine esterase activities of five plants used as Portuguese food spices. Food Chem 2007;103:778-86.

Dean, G, Ghoy H, Well Z. Streptozotocin doses and its effect. Pharmacol 1986;8[18]:121-30.

Sheneer. The blood morphology of laboratory 3rd edd; 1967.

Albarracin CA, Fuqua BC, Evans JL, Goldfine ID. Chromium picolinate and biotin combination improves glucose metabolism in treated, uncontrolled overweight to obese patients with type 2 diabetes. Diabetes Metab Res Rev 2008;24(1):41-51.

Smith DM, Pickering RM, Lewith GT. A systematic review of vanadium oral supplements for glycaemic control in type 2 diabetes mellitus. QJM 2008;101(5):351.

O’Connell BS. Selected vitamins and minerals in the management of diabetes. Diabetes Spectrum 2001;14(3):133-48.

Kaur G, Kulkarni SK. Studies on modulation of feeding behavior by atypical antipsychotics in female mice. Prog Neuro-psychopharmacol Biol Psychiatry 2002;26:277– 85.

Andrade EHA, Alves CN, GuimarÙ…es EF, Carreira LMM, Maia JGS. Variability in essential oil composition of Piper dilatatumL. C Rich Biochem Syst Ecol 2011;39:669-75.

Cho SH, Kim TH, Lee NH, Son HS, Cho IJ, Ha TY. Effects of Cassia tora fibre supplement on serum lipids in Korean diabetic patients. J Med Food 2005;8(3):311-8.

Chang LM, Jou TS, Yang WS, Wu HP, Huang SH, Tai TY, et al. Therapeuticeffect of guar gum in patients with non-insulin-dependent diabetes mellitus. J Formos Med Assoc 1992;91(1):15-9.

Gilgwn-Sherki Y, Melamed E, Offen D. Oxidative stress induced-neurodegenerative disease: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacol 2001;40:959-75.

Maggiolini M, Picard D. The unfolding stories of GPR30, a new membrane-bound estrogen receptor. J Endocrinol 2010;204(2):105–14.

Mizukami Y. In vivo functions of GPR30/GPER-1, a membrane receptor for estrogen: from discovery to functions in vivo. Endocr J 2010;57(2):101–7.

Wetherill YB. In vitro molecular mechanisms of bisphenol A. Reprod Toxicol 2007;24(2):178-98.

Martensson UE. Deletion of the G protein-coupled receptor 30 impairs glucose tolerance, reduces bone growth, increases blood pressure, and eliminates estradiol-stimulated insulin release in female mice. Endocrinol 2009;150(2):687–98.

Kathy pedcky. Aromtherapist. Importance of extranuclear estrogen receptor-alpha and membrane G protein-coupled estrogen receptor in pancreatic islet survival. Diabetes 2012;58[10]:2292–302.

Derosa G, Gaddr AV, Piccinni MN, Ciccarelli N, Salvadeo S. Antithrombotic effects of rosiglitazone metformin versus glimepiride–metformin combination therapy in patients with type II diabetes mellitus and metabolic syndrome ."Pharmacother 2007;25(5):637–45.

Nagle DG, Ferreira D, Zhou YD. Epigallocatechin-3-gallate (EGCG): chemical. Beneficial effects of high dietary fiber intake in patients with type 2 diabetes mellitus. N Engl J Med 2000;342[19]:1392-8.

Tucker, Arthur O, Thomas De Baggio. The Big Book of Herbs. Loveland, CO: Interweave; 2000.

Chandalia M, Garg A, Lutjohann D, von Bergmann K, Grundy S, Brinkley LJ. Beneficial effects of high dietary fiber intake in patients with type 2 diabetesmellitus. N Engl J Med 2000;342[19]:1392-8.

Miller ME, Byington RP, Goff DC Jr, Bigger JT. Effects of intensive glucose lowering in type 2 diabetes. N Engl J Med 2008;58[24]:2545-59.

De Sousa DP. Analgesic-like activity of essential oils constituents. Mol 2011;16:2233-52.

De Sousa DP, JÙ’nior GAS, Andrade LN, Batista JS. Spasmolytic activity of chiral monoterpene esters. Rec Nat Prod 2011;5:117-22.

Jaywalk P, Kumar P, Singh VK, Singh DK. Biological effects of myristicafragrans. Ann Rev Biomed Sci 2009;11:21-9.

Muchtaridi Subarnas A, Apriyantono A, Mustarichien R. Identification of compounds in the essential oil of nutmeg seeds (myristicafragranshoutt.) that inhibit locomotor activity in mice. Int J Mol Sci 2010;11:4771-81.



How to Cite

El-hodairy, F. A. “NEUROPROTECTIVE EFFECTS OF PIMPINELLA ANISUM ON NORMAL AND DIABEITC RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 12, Dec. 2014, pp. 9-12,