DETERMINATION OF PHENOLIC COMPONENTS AND ANTIOXIDANT ACTIVITY OF SOME EGYPTIAN TEA SAMPLES

Authors

  • Ragaa El Sheikh Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig,44519, Egypt
  • Alaa S. Amin Chemistry Department, Faculty of Sciences, Benha University, Benha, Egypt
  • Mohammed A. Atwa Food Technology Research Institute, Giza, Egypt.
  • Ayman A. Gouda Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, Egypt
  • Amira A. Abdullah Food Technology Research Institute, Giza, Egypt

Keywords:

Phenolic compounds, Flavonoids, Antioxidant activity, Egyptian tea

Abstract

Objective: Tea is the most widely consumed beverage in the world. Green tea (Camellia sinensis) is a good source of bioactive compounds and it is gaining interest due to its health benefits. The present study was conducted to determine the total polyphenols and total flavonoid content as well as the antioxidant activity of ethanolic extracts from different Egyptian tea samples.

Methods: The determination process carried out using standard spectrophotometric methods and quantified various phenolic and flavonoid compounds by using high performance liquid chromatography (HPLC) method. The comparison was also made between Egyptian tea samples and commonly tea samples in the markets.

Results: Results from this study showed that Egyptian green tea showed a significant higher values of total phenol and flavonoid, contents. The same trend was also observed in antioxidant activity as assessed using DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay. It revealed that the total antioxidant activity could be due to its total phenol content.

Conclusion: The study concludes that Egyptian green tea offers higher amount of antioxidant properties.

 

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Author Biography

Ayman A. Gouda, Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, Egypt

Pharmaceutical Analysis

References

Weisbuger JH. Tea Antioxidants and health. In: Handbook of Antioxidants, Dekker: New York; 1996.

Thieleckeaba F, Boschmann M. The potential role of green tea catechins in the prevention of the metabolicsyndrome–A review. Phytochem 2009;70:11–4.

Balentine DA. Manufacturing and chemistry of tea, in phenolics compounds in food and their effects of health: Amer Chem Soc Washington, DC; 1992.

Wiseman SA, Balentine DA, Frei B. Antioxidants in tea. Crit Rev Food Sci Nutr 1997;37:705-8.

Kavanagh KT, Hafer LJ, Kim DW, Mann KK, Sherr DH, Rogers AE, et al. Green tea extracts decrease carcinogeninduced mammary tumor burden in rats and rate of breast cancer cell proliferation in culture. J Cell Biochem 2001;82:387-98.

Koo SI, Noh SK. Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect. J Nutri Biochem 2007;18:179-83.

Farhoosh R, Golmovahhed GA, Khodaparast MHH. Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chem 2007;100:231–6.

Wang H, Helliwell K. Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography. Food Res Inter 2001;34:223–7.

Chan EWC, Lim YY, Chew YL. Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food Chem 2007;102:1214–22.

Pilar AM, Carbó R, Jiménez JAL, Gordon MH. Antioxidant and microbial activities of tea infusions. Food Chem 2008;108:55–63.

Leung LK, Su YL, Chen RY, Zhang ZS, Huang Y, Chen ZY. Theaflavins in black tea and catechins in green tea are equally effective antioxidants. J Nutr 2001;131:2248–51.

Roghelia V, Patel VH. Antioxidant profile of organic and conventional grown green tea (Camellia Sinensis). IJBPAS 2013;2:1141-50.

Nor Qhairul Izzreen MN, Mohd Fadzelly AB. Phytochemicals and antioxidant properties of different parts of Camellia sinensis leaves from Sabah Tea Plantation in Sabah, Malaysia. Int Food Res J 2013;20:307-12.

Turkmen N, Sari F, Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods. Food Chem 2006;99:835–41.

Lena MG, Philip JB, Chee SY. Examination of antioxidant activity of Ginkgo biloba leaf infusions. Food Chem 2003;82:275–82.

Templer SJ, Boctel AJB. A comparison of dryer types used for tea drying. J Agric Engin Res 2000;77:401–6.

Amico V, Chillemi R, Mangiafico S, Spatafora C, Tringali C. Polyphenol-enriched fractions from Sicilian grape pomace: HPLC DAD analysis and antioxidant activity. Bioresour Technol 2008;99:5960-6.

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.

Goupy P, Hugues M, Biovia P, Amiot MJ. Antioxidant composition and activity of barley (Hordeum vulgare) and malt extracts and of isolated phenolic compounds. J Sci Food Agric 1999;79:1625-34.

Leong LP, Shui G. An investigation of antioxidantcapacity of fruits in Singapore markets. Food Chem 2002;76:69-75.

Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT-Food Sci Techn 1999;32:269-77.

Ling JJ, Mohamed M, Rahmat A, Abu Bakar MF. Phytochemicals, antioxidant properties and anticancer investigations of the different parts of several gingers species (Boesenbergia rotunda, B. pulchella var attenuata and B. armeniaca). J Med Plants Res 2010;4:27-32.

Pilar AM, Carbó R, Jiménez JAL, Gordon MH. Antioxidant and microbial activities of tea infusions. Food Chem 2008;108:55–63.

Published

01-04-2015

How to Cite

El Sheikh, R., A. S. Amin, M. A. Atwa, A. A. Gouda, and A. A. Abdullah. “DETERMINATION OF PHENOLIC COMPONENTS AND ANTIOXIDANT ACTIVITY OF SOME EGYPTIAN TEA SAMPLES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 4, Apr. 2015, pp. 198-02, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/4685.

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