PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY OF PURE SYRINGIN IN COMPARISON TO VARIOUS SOLVENTS EXTRACTS OF MUSA PARADISIACA (BANANA) (FRUIT AND FLOWER) AND TOTAL PHENOLIC CONTENTS

Authors

  • Bashir Ado Ahmad Faculty of Medicine, Universiti Sultan Zainal Abidin.
  • Khamsah Suryati Mohd Faculty of Bioresource and Food Industry, Universiti Sultan Zainal Abidin.
  • Muhammad Abdurrazak Faculty of Medicine, Universiti Sultan Zainal Abidin.
  • U. S Mahadeva Rao Faculty of Medicine, Universiti Sultan Zainal Abidin.
  • Thant Zin Universiti Medical Lecturer

Keywords:

Banana, Radical scavenging, Tepal, Syringin

Abstract

Objective: The study concentrated on exploring the radical scavenging activity of various banana extracts in comparison to pure syringin and the total phenolic contents of the extracts.

Methods: The banana parts were prepared and extracted by cold extraction technique and the extracts obtained were used to carry out some phytochemical screening by Trease and Evans method. The ability of the extract to scavenge free radicals was measured using 2, 2-diphenyl-1-pcrylhydrazyl radical using quercetin as a reference radical scavenger by the method of Gyamfi. Further analysis of total phenolic contents present in the extracts was carried out using Singleton and Rossi method.

Results: Tepal methanol extract was found to have the highest radical scavenging activity compared to others, such as tepal ethanol, tepal aqueous, skin methanol, flesh methanol and pure syringin. The IC50 value of the tepal methanol extract was found to be 22.5 µg/ml. The highest total phenolic contents (expressed as microgram of Gallic acid equivalent per gram of the extracts) were found in tepal methanol extract (8000 µg/g) and the least in Flesh methanol extract (2150 µg/g).

Conclusion: The results generated from this study showed that tepal banana extracts possess very good radical scavenging activity and as well the largest amount of phenolic contents, which could introduce phenols as the main radical scavenger in banana extracts and offering effective protection from free radicals, and the antioxidant activity of pure syringin was not obvious may be due to the presence of highly anti oxidative compounds present in banana, phenolics and flavonoids.

 

Downloads

Download data is not yet available.

Author Biographies

Bashir Ado Ahmad, Faculty of Medicine, Universiti Sultan Zainal Abidin.

Masters' students

Khamsah Suryati Mohd, Faculty of Bioresource and Food Industry, Universiti Sultan Zainal Abidin.

Lecturer

Muhammad Abdurrazak, Faculty of Medicine, Universiti Sultan Zainal Abidin.

Masters' students

U. S Mahadeva Rao, Faculty of Medicine, Universiti Sultan Zainal Abidin.

Universiti Medical Lecturer

References

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, Metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160:1-40.

Mandal S, Yadav S, Yadav S, Nema RK. Antioxidants: a review. J Chem Pharm Res 2009;1(1):102-4.

Ajitha M, Rajnarayana K. Role of oxygen free radicals in human disease. Indian Drugs 2001;38:545-54.

Halliwell B, Aeschbach R, Loliger J, Aruom OI. The Characterization of antioxidants. Food Chem Toxicol 1995;33:601-17.

Asolkar L, Kallar KK, Chakre OJ. Glossary of Indian medicinal plants with active principles, Publications and information directorate. 2nd Ed. New Delhi; 1992. p. 72-3.

Chemexcil. Selected Medicinal Plants of India. Basic Chemicals, Pharmaceutical and Cosmetic Export Promotion Council, Bombay, India; 1992. p. 56.

Yin X, Quan J, Kanazawa T. Banana prevents plasma oxidative stress in healthy individuals. Plant Foods Hum Nutr 2008;63:71-6.

Mokbel MS, Hashinaga F. Antibacterial and antioxidant activities of banana (Musa, AAA CV. Cavendish) Fruits Peel. Am J Biochem Biotechnol 2005;1(3):125-31.

Vijayakumar S, Presannakumar G, Vijayalakshmi NR. Antioxidant activity of banana flavonoids. Fitoterapia 2008;79:279-82.

Lian Y, Yan HY, Ming-Yan W, Zhang, Jin-a D. Effects of Syringin from Phellodendron Chinensis on monosodium urate crystal induced inflammation and intracellular adhesion Molecule-1 (ICAM-1) expression. Afr J Pharm Pharmacol 2012;6 (21):1515-9.

Nakazawa T, Yasuda T, Ohsawa K. Metabolites of orally administered Magnolia Officinalis extract in rats and man and its antidepressant like effects in mice. J Pharm Pharmacol 2003;55:1583-91.

Eleni HP, Paula MF, Supriy K. Stimulation of adhesion molecule expression in human endothelial cells (HUVECs) by adrenomodulin and corticotrophin. Am J Physiol Cell Physiol 2004;286:239-46.

Nour-Eddine E, Kollman A, Khlifi S, Ducrot PH. Antioxidative effect of compounds isolated from Globularia alypum L, Structure 3/activity Relationship. Food Sci Technol 2007;40:1246-52.

Niu HS, Hsu FL, Liuc IM. Role of sympathetic tone in the loss of syringin induced plasma glucose lowering action in conscious wistar rats. Neurosci Lett 2008;445:113-6.

Wan Z, Jiang SC, Zhu Y. Isolation and Anti-tumor effect of syringin from Acanthopanax Senticosu. Shi Zhen Med Mater Med Res 2010;21:752-3.

Shanmuga SCK, Iyyam PS, Sorimuthu PS. Isolation, Characterization of Syringin, Phenylpropanoid Glycoside from Musa Paradisiaca tepal extract and evaluation of its antidiabetic effect in streptozotocin-induced diabetic rats. Biomed Prev Nutr 2014;4:105-11.

Trease GE, Evans WC. Trease and Evan’s Textbook of Pharmacognosy. 13th Ed. London: Cambridge University Press; 1989. p. 546.

Gyamfi MA, Yonamine M, Aniya Y. Free radical scavenging action of medicinal herbs from ghana: Thonningia sanguinea on experimentally induced liver injuries. Gen Pharmacol 1999;32:661-7.

Krings U, Berger RG. Antioxidant activity of some roasted foods. Food Chem 2001;72:223-9.

Singleton VL, Rossi Jr JA. Colorimetry of total phenolics with Phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.

Lewis DL, Field WD, Shaw GP. A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. Var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. J Ethnopharmacol 1999;65:283–8.

Lewis DA, Shaw GP. A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions. J Nutr Biochem 2001;12:95-100.

Ragasa CY, Martinez A, Chua JEY, Rideout JA. A Triterpene from Musa errans. Philipp J Sci 2007;136(2):167-71.

Ghani A. Medicinal plants of bangladesh: chemical constituents and uses. 2nd Ed. The Asiatic Society of Bangladesh, Dhaka, Bangladesh; 2003. p. 315.

Ehiowemwenguan G, Emoghene AO, Inetianbor JE. Antibacterial and Phytochemical analysis of banana fruit peel. IOSR J Pharm 2014;4(8):18-25.

Mallikarjuna R, Prasad SHKR, Jyothirmayi N. Efficacy of Ripened and Unripened fruit extracts of Musa X Paradisiaca L. (Bontha Cultivar) against human pathogens. Int J Pharm Pharm Sci 2012;4(1):457-8.

Jini J, David P, Kavitha MP, Dineshkumar B, Jalaja Menon S, Bhat AR, et al. Preliminary phytochemical screening and in vitro antioxidant activity of banana flower (Musa paradisiaca AAB Nendran variety). J Pharm Res 2014;8(2):144-7.

Mohammad I, Zafar I, Javid H, Hidayat H, Manzoor A, Asma E, et al. Chemical constituents and antioxidant activity of Geranium Wallichianum. Rec Nat Prod 2009;3(4):193-7.

Bondet V, Brand-Williams W, Berset C. Kinetics and mechanisms of antioxidant activity using the DPPH• free radical method. Food Sci Technol 1997;30:609-15.

Iranshahi M, Askari M, Sahebkar A, Hadjipavlou-Litina D. Evaluation of Antioxidant, Anti-inflammatory and lipoxygenase inhibitory activities of the prenylated coumarin umbelliprenin. DARU 2009;17(2):99-103.

Tosun M, Ercisli S, Sengul M, Ozer H, Polat T. Antioxidant properties and total phenolic content of eight salvia species from turkey. Biol Res 2009;41:175-81.

Roya K, Fatemeh G. Screening of total phenol and flavonoid content, Antioxidant and antibacterial activities of the methanolic extracts of three Silene species from Iran. Int J Agric Crop Sci 2013;5(3):305-12.

Shahidi F, Janitha PK, Wanasundara PD. Phenolic Antioxidants. CRC Critical Rev Food Sci Nutr 1992;32(1):67-103.

Osawa T. Novel natural antioxidants for utilization in food and biological systems. Post-harvest biochemistry of plant food materials in the tropics. Japan Scientific Societies Press; 1994. p. 241-51.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.

Tanaka M, Kuie CW, Nagashima Y, Taguchi T. Applications of antioxidative maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi 1988;54:1409–14.

Published

01-05-2015

How to Cite

Ahmad, B. A., K. S. Mohd, M. Abdurrazak, U. S. M. Rao, and T. Zin. “PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY OF PURE SYRINGIN IN COMPARISON TO VARIOUS SOLVENTS EXTRACTS OF MUSA PARADISIACA (BANANA) (FRUIT AND FLOWER) AND TOTAL PHENOLIC CONTENTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 242-6, https://www.innovareacademics.in/journals/index.php/ijpps/article/view/5007.

Issue

Section

Original Article(s)